Lefort Craig T, Hyun Young-Min, Kim Minsoo
Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY, USA.
Methods Mol Biol. 2012;757:205-14. doi: 10.1007/978-1-61779-166-6_14.
Aberrant integrin activation is associated with several immune pathologies. In leukocyte adhesion deficiency (LAD), the absence or inability of β(2) integrins to undergo affinity upregulation contributes to recurrent infectious episodes and impaired wound healing, while excessive integrin activity leads to an exaggerated inflammatory response with associated tissue damage. Therefore, integrin activation is an attractive target for immunotherapies, and monitoring the effect of agents on integrin activation is necessary during preclinical drug development. The activation of integrins involves the structural rearrangement of both the extracellular and cytoplasmic domains. Here, we describe methods for monitoring integrin conformational activation using fluorescence resonance energy transfer (FRET).
异常的整合素激活与多种免疫病理相关。在白细胞黏附缺陷症（LAD）中，β(2)整合素缺乏或无法进行亲和力上调会导致反复感染发作和伤口愈合受损，而整合素活性过高则会导致炎症反应过度并伴有相关组织损伤。因此，整合素激活是免疫治疗的一个有吸引力的靶点，并且在临床前药物开发过程中监测药物对整合素激活的影响是必要的。整合素的激活涉及细胞外和细胞质结构域的结构重排。在这里，我们描述了使用荧光共振能量转移（FRET）监测整合素构象激活的方法。
