盘基网柄菌趋化性分析。
Analysis of chemotaxis in Dictyostelium.
作者信息
Cai Huaqing, Huang Chuan-Hsiang, Devreotes Peter N, Iijima Miho
机构信息
Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
出版信息
Methods Mol Biol. 2012;757:451-68. doi: 10.1007/978-1-61779-166-6_26.
Abstract
Dictyostelium discoideum is an excellent model organism for the study of directed cell migration, since Dictyostelium cells show robust chemotactic responses to the chemoattractant cAMP. Many powerful experimental tools are applicable, including forward and reverse genetics, biochemistry, microscopy, and proteomics. Recent studies have demonstrated that many components involved in chemotaxis are functionally conserved between human neutrophils and Dictyostelium amoebae. In this chapter, we describe how to define the functions of proteins that mediate and regulate cell motility, cell polarity, and directional sensing during chemotaxis in Dictyostelium.
摘要
盘基网柄菌是研究定向细胞迁移的优秀模式生物，因为盘基网柄菌细胞对趋化因子cAMP表现出强烈的趋化反应。许多强大的实验工具都适用，包括正向和反向遗传学、生物化学、显微镜学和蛋白质组学。最近的研究表明，许多参与趋化作用的成分在人类中性粒细胞和盘基网柄菌变形虫之间功能保守。在本章中，我们描述了如何确定在盘基网柄菌趋化过程中介导和调节细胞运动、细胞极性和方向感知的蛋白质的功能。
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