Lindh E, Frederiksen W
Department of Medical Microbiology, University of Lund, Malmö General Hospital, Sweden.
APMIS. 1990 Apr;98(4):358-62.
Genotypic relatedness was assessed to clarify the taxonomic position of strains phenotypically behaving like K. pneumoniae, but for the ornithine reaction. Using DNA-DNA hybridization it could be shown that 25 non-motile ornithine decarboxylating strains showed high genotypic relatedness to the type strain of K. pneumoniae. Thus, it is proposed that they be considered as ornithine decarboxylating strains of the species K. pneumoniae. The API 20E system was used for phenotypic characterization, but the API code obtained by these strains was not registered in the API Profile Index. However, except for the ornithine reaction the isolates behaved as typical K. pneumoniae. Three ornithine negative strains of E. aerogenes were identified as K. pneumoniae by the API 20E System, but they showed high genotypic relatedness to the type strain of E. aerogenes.
评估基因型相关性以阐明表型上类似肺炎克雷伯菌但鸟氨酸反应不同的菌株的分类地位。通过DNA-DNA杂交表明，25株无动力鸟氨酸脱羧菌株与肺炎克雷伯菌模式菌株显示出高度的基因型相关性。因此，建议将它们视为肺炎克雷伯菌的鸟氨酸脱羧菌株。使用API 20E系统进行表型特征分析，但这些菌株获得的API编码未在API Profile Index中登记。然而，除鸟氨酸反应外，分离株表现为典型的肺炎克雷伯菌。三株产气肠杆菌鸟氨酸阴性菌株通过API 20E系统被鉴定为肺炎克雷伯菌，但它们与产气肠杆菌模式菌株显示出高度的基因型相关性。
