Thorfinn J, Angelidis I K, Gigliello L, Pham H M, Lindsey D, Chang J
Section of Plastic Surgery, Department of Veterans Affairs and Division of Plastic Surgery, Stanford University Medical Center, Stanford, CA, USA.
J Hand Surg Eur Vol. 2012 Feb;37(2):109-14. doi: 10.1177/1753193411419439. Epub 2011 Sep 15.
Tissue-engineered rabbit flexor tendons reseeded with cells are stronger in vitro after culture in a bioreactor. It is not known whether this effect persists in vivo. Tenocytes from New Zealand white rabbits were seeded onto rabbit rear paw flexor tendons that were deprived of cells and exposed to cyclic strain in a bioreactor. Reseeded constructs that were kept unloaded in a medium for 5 days were used as controls. The tendons were implanted to bridge a zone II defect in the rabbit. After explantation 4 weeks later, the ultimate tensile strength (UTS) and elastic modulus (EM) were determined. Tendon constructs that were exposed to cyclic strain had significantly improved UTS and EM. Histology showed that cellularity was increased in the bioreactor tendons.
在生物反应器中培养后，重新接种细胞的组织工程化兔屈肌腱在体外更强壮。尚不清楚这种效应在体内是否持续存在。将来自新西兰白兔的肌腱细胞接种到去除细胞并在生物反应器中承受循环应变的兔后爪屈肌腱上。将在培养基中无负荷保存5天的重新接种构建体用作对照。将肌腱植入以桥接兔的II区缺损。4周后取出，测定极限拉伸强度（UTS）和弹性模量（EM）。承受循环应变的肌腱构建体的UTS和EM显著改善。组织学显示生物反应器中的肌腱细胞增多。
