School of Traditional Chinese Materia Medica 48#, Shenyang Pharmaceutical University, Wenhua Road 103, Shenhe District, Shenyang 110016, P. R. China.
Chem Biodivers. 2011 Sep;8(9):1682-8. doi: 10.1002/cbdv.201000289.
Two novel flavanone glycosides, homoeriodictyol 7-O-β-D-[6-(3-hydroxybutanoyl)glucopyranoside] (viscumneoside IX; 1) and homoeriodictyol 7-O-β-D-6-(3-hydroxybutanoyl)glucopyranosyl-β-D-glucopyranoside (viscumneoside X; 2), together with four known flavanoids, 2-homoeriodictyol 7-O-β-D-glucopyranoside (3), viscumneoside I (4), viscumneoside III (5), and 4',5-dihydroxy-3'-methoxy-7-(2-O-α-L-rhamnopyranosyl-β-D-glucopyranosyloxy)flavanone (6) were isolated from stems and leaves of Viscum coloratum. Their structures were elucidated on the basis of their NMR spectra, HR-FAB-MS data, and acid hydrolysis. Inhibitory effects of the four compounds 1-4 on the formation of osteoclast-like multinucleated cells were investigated. As a result, all the four flavanoids showed significant inhibitory effects on the formation of osteoclast-like multinuclear cells even at a low concentration of 2 μg/ml. The activities of 1-4 at such a concentration exceeded or approximated to that of elcitonin, the positive control drug at a concentration of 2 U/ml, suggesting that they may be of interest for the development of new anti-osteoporosis drugs.
从槲寄生的茎叶中分离得到了两个新的黄烷酮糖苷，即 homoeriodictyol 7-O-β-D-[6-(3-hydroxybutanoyl)glucopyranoside]（viscumneoside IX；1）和 homoeriodictyol 7-O-β-D-6-(3-hydroxybutanoyl)glucopyranosyl-β-D-glucopyranoside（viscumneoside X；2），以及四个已知的黄烷酮化合物，2-homoeriodictyol 7-O-β-D-glucopyranoside（3）、viscumneoside I（4）、viscumneoside III（5）和 4',5-二羟基-3'-甲氧基-7-(2-O-α-L-鼠李吡喃糖基-β-D-葡萄糖吡喃糖苷基氧基)黄烷酮（6）。它们的结构是根据其 NMR 光谱、高分辨率-FAB-MS 数据和酸水解确定的。研究了这四个化合物 1-4 对破骨细胞样多核细胞形成的抑制作用。结果表明，四种黄烷酮化合物在低浓度 2μg/ml 时，对破骨细胞样多核细胞的形成均有明显的抑制作用。在如此浓度下，1-4 的活性超过或接近阳性对照药物 elcitonin（浓度为 2U/ml），表明它们可能对开发新的抗骨质疏松药物具有重要意义。
