Xu Liang-Jie, Yan Jin-Chuan, Wang Biao, Liu Pei-Jing, Gong Jie, Wang Cui-Ping
Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2011 Jun;39(6):526-30.
To investigate the effect of OX40/OX40L interaction on the nuclear factor of activated T cells c1 (NFATc1) in ApoE-/- mice.
Lymphocytes were prepared from mouse spleens after Collar-treated Surgery, then incubated with a range of agonistic anti-OX40 mAbs and inhibitory anti-OX40L mAb to stimulate or inhibit OX40-OX40L interaction in vitro. The expression of NFATc1 mRNA and protein in lymphocytes of ApoE-/- mice was measured by Real Time PCR and flow cytometry, respectively.
(1) After stimulating OX40-OX40L signal pathway, the expression of NFATc1 mRNA and protein in leukocytes of ApoE-/- mice was significantly increased, with maximal effect occurring at 20 µg/ml anti-OX40 mAb-stimulated, and peaked at 24 h at any concentration (P < 0.01). (2) Anti-OX40L mAb significantly suppressed the expression of NFATc1 in leukocytes of ApoE-/- mice, with maximal effect occurring at 20 µg/ml anti-OX40L mAb, and peaked at 24 h (P < 0.001).
OX40-OX40L interaction can regulate the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE-/- mice.
研究OX40/OX40L相互作用对载脂蛋白E基因敲除（ApoE-/-）小鼠活化T细胞核因子c1（NFATc1）的影响。
经颈圈处理手术后从小鼠脾脏制备淋巴细胞，然后与一系列激动性抗OX40单克隆抗体和抑制性抗OX40L单克隆抗体孵育，以在体外刺激或抑制OX40 - OX40L相互作用。分别通过实时聚合酶链反应（Real Time PCR）和流式细胞术检测ApoE-/-小鼠淋巴细胞中NFATc1 mRNA和蛋白的表达。
（1）刺激OX40 - OX40L信号通路后，ApoE-/-小鼠白细胞中NFATc1 mRNA和蛋白的表达显著增加，在20μg/ml抗OX40单克隆抗体刺激时效果最佳，且在任何浓度下均在24小时达到峰值（P < 0.01）。（2）抗OX40L单克隆抗体显著抑制ApoE-/-小鼠白细胞中NFATc1的表达，在20μg/ml抗OX40L单克隆抗体时效果最佳，并在24小时达到峰值（P < 0.001）。
OX40 - OX40L相互作用可调节ApoE-/-小鼠淋巴细胞中NFATc1的mRNA和蛋白表达。
