Department of Nutritional Sciences, College of Human Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
Food Chem Toxicol. 2011 Dec;49(12):3074-80. doi: 10.1016/j.fct.2011.09.001. Epub 2011 Sep 10.
Shiitake mushrooms (SMs) have been used in Asia for treatment and/or prevention of chronic diseases and hypercholesterolemia. Previously, we observed a diet supplemented with 5% SM resulted in a twofold increase in plasma IL-6 levels in DBA arthritic mice. An elevation in plasma IL-6 has also been implicated in the pathogenesis fatty liver disease. Thus, the aim of this study was to investigate the effect of SM supplemented-diet on hepatic steatosis. In study 1, eight-week old female C57BL/6 mice were randomly assigned to the following groups for 6 weeks: the AIN-93 diet; 5% SM, and 5% white button mushroom (WBM) supplemented diets (12/group). In study 2, mice were fed either the AIN-93 diet or SM (20/group). After 6 weeks, 13 mice fed SM diet were given the AIN93 diet for 8 or 15 days. Unlike other groups, all mice fed the SM diet developed fatty liver (mean histopathology score 4.5 vs <1 in the other groups; p<0.001) without fibrosis and inflammation. Fifteen days post withdrawal of SM completely normalized liver histology. To the best of our knowledge, this is the first report that chronic consumption of SM is associated with the development of fatty liver. The mechanism by which SM causes hepatic steatosis warrants further investigation.
香菇（SM）在亚洲被用于治疗和/或预防慢性病和高胆固醇血症。此前，我们观察到，饮食中补充 5%的 SM 会使 DBA 关节炎小鼠的血浆 IL-6 水平增加一倍。血浆 IL-6 的升高也与脂肪肝疾病的发病机制有关。因此，本研究旨在研究 SM 补充饮食对肝脂肪变性的影响。在研究 1 中，将 8 周龄雌性 C57BL/6 小鼠随机分为以下几组，喂养 6 周：AIN-93 饮食；5%SM 和 5%白蘑菇（WBM）补充饮食（每组 12 只）。在研究 2 中，将小鼠分别喂养 AIN-93 饮食或 SM（每组 20 只）。6 周后，13 只喂养 SM 饮食的小鼠给予 AIN93 饮食 8 或 15 天。与其他组不同，所有喂养 SM 饮食的小鼠均出现脂肪肝（平均组织病理学评分 4.5 与其他组相比<1；p<0.001），无纤维化和炎症。停止 SM 喂养 15 天后，肝脏组织学完全恢复正常。据我们所知，这是第一个报道慢性食用 SM 与脂肪肝发展有关的报告。SM 引起肝脂肪变性的机制需要进一步研究。
