Immuno-dermatology laboratory, Onco-dermatology unit, CIC biothérapie INSERM 0503, CHU Hôtel-Dieu, 1 place A. Ricordeau, 44093 Nantes Cedex 1, France.
Eur J Dermatol. 2011 Nov-Dec;21(6):899-905. doi: 10.1684/ejd.2011.1526.
There is increasing evidence that melanoma cells express TLR2, -3, and -4. However, the expression of other TLRs by these cells still remains unknown. We investigated the expression patterns of TLR2, -3, -4, -7, -8 and -9 both on melanoma-invaded lymph nodes and on melanoma cell lines. TLR2, -3, -4, -7 and -9 mRNA expression was determined by quantitative RT-PCR. The TLR protein expression level was measured ex vivo by immunohistochemistry and in vitro by flow cytometry.
At the mRNA level, melanoma cells in vitro, and possibly ex vivo, expressed TLR2, -3, -4, -7 and -9. TLR2 and -4 protein expressions ex vivo were over 50%, contrasting with an absence of these 2 TLRs in vitro. On the contrary, TLR-3 and -8 proteins had a low expression ex vivo with a high expression in vitro. TLR-7 and -9 proteins were expressed ex vivo and in vitro. Our study demonstrates for the first time that melanoma cells express TLR7 and -8.
黑色素瘤细胞表达 TLR2、-3 和 -4，本研究旨在检测黑色素瘤细胞表达其他 TLR 的情况。
通过实时定量 RT-PCR 检测 TLR2、-3、-4、-7、-8 和 -9 在黑色素瘤浸润淋巴结和黑色素瘤细胞系中的表达模式。采用免疫组化和流式细胞术检测 TLR 蛋白的表达水平。
体外和可能的体外实验均表明，黑色素瘤细胞在 mRNA 水平上表达 TLR2、-3、-4、-7 和 -9。TLR2 和 -4 蛋白的表达在体外超过 50%，而在体外则不存在这两种 TLR。相反，TLR-3 和 -8 蛋白在体外表达较低，而在体外表达较高。TLR-7 和 -9 蛋白在体外和体外均有表达。本研究首次证明黑色素瘤细胞表达 TLR7 和 -8。
