Institute of Physiological Chemistry and Pathobiochemistry, Muenster University Hospital, Waldeyerstrasse 15, D-48149 Münster, Germany.
J Cell Biochem. 2012 Jan;113(1):340-8. doi: 10.1002/jcb.23360.
Cell volume is regulated by a delicate balance between ion distribution across the plasma membrane and the osmotic properties of intra- and extracellular components. Using a fluorescent calcein indicator, we analysed the effects of glycosaminoglycans on the cell volume of hyaluronan producing fibroblasts and hyaluronan deficient HEK cells over a time period of 30 h. Exogenous glycosaminoglycans induced cell blebbing after 2 min and swelling of fibroblasts to about 110% of untreated cell volume at low concentrations which decreased at higher concentrations. HEK cells did not show cell blebbing and responded by shrinking to 65% of untreated cell volume. Heparin induced swelling of both fibroblasts and HEK cells. Hyaluronidase treatment or inhibition of hyaluronan export led to cell shrinkage indicating that the hyaluronan coat maintained fibroblasts in a swollen state. These observations were explained by the combined action of the Donnan effect and molecular crowding.
细胞体积通过跨质膜的离子分布和细胞内外成分的渗透性质之间的微妙平衡来调节。使用荧光钙黄绿素指示剂，我们分析了糖胺聚糖对透明质酸产生的成纤维细胞和透明质酸缺乏的 HEK 细胞体积的影响，时间跨度为 30 小时。外源性糖胺聚糖在 2 分钟后诱导细胞起泡，并在低浓度下使成纤维细胞膨胀至未处理细胞体积的约 110%，在较高浓度下则降低。HEK 细胞没有出现细胞起泡现象，而是收缩至未处理细胞体积的 65%。肝素诱导成纤维细胞和 HEK 细胞肿胀。透明质酸酶处理或抑制透明质酸外排导致细胞收缩，表明透明质酸涂层使成纤维细胞保持肿胀状态。这些观察结果可以通过唐南效应和分子拥挤的联合作用来解释。
