一种用于β-肾上腺素能受体表征的抗生物素蛋白-生物素化普萘洛尔复合物。

An avidin-biotinyl-propranolol complex for beta-adrenergic receptor characterization.

作者信息

Meier K E, Ruoho A E

出版信息

J Supramol Struct. 1978;9(2):243-52. doi: 10.1002/jss.400090209.

DOI:10.1002/jss.400090209

Abstract

The synthesis of biotinyl-hexaglycyl-NEDA (abbreviation:BGN), a biotinyl derivative of propranolol, is described. This bifunctional molecule binds with high affinity to the biotin-binding protein, avidin. The duck erythrocyte was used as a model beta-receptor system. Formation of an avidin-BGN-beta-receptor complex was demonstrated in intact erythrocytes, in erythrocyte ghosts, and in the digitonin-solubilized beta-receptor. The avidin-BGN complex will be used for localization and purification of the beta-receptor.

摘要

描述了普萘洛尔的生物素衍生物生物素基-六甘氨酰-NEDA（缩写：BGN）的合成。这种双功能分子与生物素结合蛋白抗生物素蛋白具有高亲和力。鸭红细胞被用作β受体系统模型。在完整红细胞、红细胞血影以及洋地黄皂苷增溶的β受体中均证实了抗生物素蛋白-BGN-β受体复合物的形成。抗生物素蛋白-BGN复合物将用于β受体的定位和纯化。

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引用本文的文献

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Iodoazidobenzylpindolol, a photoaffinity probe for the beta-adrenergic receptor.碘叠氮苄基吲哚洛尔，一种β-肾上腺素能受体的光亲和探针。

Proc Natl Acad Sci U S A. 1981 Mar;78(3):1609-13. doi: 10.1073/pnas.78.3.1609.

2

Biochemical characterization of the beta-adrenergic receptor of the frog erythrocyte.青蛙红细胞β-肾上腺素能受体的生化特性

Mol Cell Biochem. 1979 Dec 14;28(1-3):45-66. doi: 10.1007/BF00223359.

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