Dhople A M, Ortega I
Dept. of Biological Sciences, Florida Institute of Technology, Melbourne 32904.
Indian J Lepr. 1990 Jan-Mar;62(1):66-75.
An in vitro culture system has been devised for the maintenance and growth of M. leprae in a cell-free medium. Cells from four-week old cultures could be transferred to fresh medium and normal growth was observed in subcultures. Using this system, the M.I.Cs of dapsone and rifampicin were determined. Dapsone at 25 ng/ml and rifampicin at 300 ng/ml completely inhibited the growth of host-grown as well as in vitro-adapted M. leprae. It was further shown that the effects of both the drugs were bactericidal; this observation was subsequently confirmed using mouse foot-pad technique.
已设计出一种体外培养系统，用于在无细胞培养基中维持和培养麻风分枝杆菌。四周龄培养物中的细胞可转移至新鲜培养基中，传代培养中观察到正常生长。利用该系统，测定了氨苯砜和利福平的最低抑菌浓度。25纳克/毫升的氨苯砜和300纳克/毫升的利福平可完全抑制宿主来源以及体外适应的麻风分枝杆菌的生长。进一步表明这两种药物的作用均具有杀菌性；随后使用小鼠足垫技术证实了这一观察结果。
