Dyková Iva, Lorenzo-Morales Jacob, Kostka Martin, Valladares Basilio, Pecková Hana
Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
Dis Aquat Organ. 2011 Jul 12;95(3):225-31. doi: 10.3354/dao02361.
A total of 109 sea urchins from 3 species collected in 2 localities off the coast of Tenerife Island, Spain, were examined for the presence of free-living amoebae in their coelomic fluid. Amoeba trophozoites were isolated exclusively from moribund individuals of long-spined sea urchins Diadema aff. antillarum (Philippi) (Echinoidea, Echinodermata) that manifested lesions related to sea urchin bald disease on their tests (16 out of 56 examined). No amoebae were detected in Arbacia lixula (L.) and Paracentrotus lividus (Lamarck). From the former sea urchin species, 8 strains, established from 10 primary isolates, were identified as Neoparamoeba branchiphila Dyková et al., 2005 using morphological and molecular methods. Results of this study (limited to the screening for free-living amoebae) together with data on agents of sea urchin mortalities reported to date justify the hypothesis that free-living amoebae play an opportunistic role in D. aff. antillarum mortality. The enlargement of the dataset of SSU rDNA sequences brought new insight into the phylogeny of Neoparamoeba species.
对从西班牙特内里费岛海岸附近两个地点采集的3种共109只海胆进行了检查，以检测其体腔液中是否存在自由生活的变形虫。变形虫滋养体仅从长刺海胆Diadema aff. antillarum（Philippi）（棘皮动物门，海胆纲）的濒死个体中分离出来，这些个体的外壳上出现了与海胆秃病相关的病变（在56只检查的个体中有16只）。在短刺海胆Arbacia lixula（L.）和紫球海胆Paracentrotus lividus（Lamarck）中未检测到变形虫。从前者海胆物种中，利用形态学和分子方法，从10个原始分离株中建立了8个菌株，鉴定为2005年Dyková等人发现的嗜鳃新帕拉变形虫（Neoparamoeba branchiphila）。本研究结果（仅限于对自由生活变形虫的筛查）以及迄今为止报道的海胆死亡病原体数据支持了以下假设：自由生活变形虫在Diadema aff. antillarum死亡中起机会性作用。小亚基核糖体DNA（SSU rDNA）序列数据集的扩大为新帕拉变形虫属物种的系统发育带来了新的见解。
