Department of Optics, Faculty of Sciences, University of Granada, Granada, Spain.
Cornea. 2011 Dec;30(12):1428-35. doi: 10.1097/ICO.0b013e31821bdfd4.
To examine the transparency characteristics at different times of development in the culture of 2 different types of human corneal stroma substitutes generated by tissue engineering using human fibrin or human fibrin and 0.1% agarose, with human keratocytes entrapped within.
The transparency of these artificial corneal stromas was analyzed after 1, 7, 14, 21, and 28 days of development in culture by determining the scattering and absorption coefficients from the spectral reflectance data of each sample using the Kubelka-Munk equations.
The scattering coefficient of both types of bioengineered tissues tended to increase with culture time and wavelength until 550 nm, whereby a slight decrease was observed for longer wavelengths. In general, the spectral distribution of the Kubelka-Munk scattering coefficient of the fibrin-agarose corneal constructs was more stable than that of the fibrin constructs. The absorption coefficient of the human fibrin and fibrin-agarose corneal substitutes tended to decrease with increasing wavelength, and their absolute values were higher for fibrin-agarose than for fibrin scaffolds, especially for short wavelengths. In addition, the spectral transmittance behavior of both types of tissue analyzed was similar to the ones of the theoretical and control corneas, with absolute values above 90% for all wavelengths at 28 days of development.
The transparency, scattering, and absorption of both fibrin and fibrin-agarose corneal stroma substitutes indicate that these new biomaterials could be adequate for clinical use.
研究使用人纤维蛋白或人纤维蛋白和 0.1%琼脂糖包埋人角膜基质细胞的组织工程方法培养的 2 种不同类型人角膜基质替代物在不同发育时间的透明度特征。
通过用 Kubelka-Munk 方程从每个样本的光谱反射率数据确定散射和吸收系数，分析这些人工角膜基质在培养 1、7、14、21 和 28 天后的透明度。
两种类型的生物工程组织的散射系数均随培养时间和波长而增加，直至 550nm，此后较长波长下观察到略有下降。一般来说，纤维蛋白-琼脂糖角膜构建物的 Kubelka-Munk 散射系数的光谱分布比纤维蛋白构建物更稳定。人纤维蛋白和纤维蛋白-琼脂糖角膜替代物的吸收系数随波长的增加而降低，其绝对值对于纤维蛋白-琼脂糖高于纤维蛋白支架，尤其是短波长。此外，分析的两种组织的光谱透过率行为与理论和对照角膜相似，在培养 28 天后，所有波长的绝对值均高于 90%。
纤维蛋白和纤维蛋白-琼脂糖角膜基质替代物的透明度、散射和吸收表明这些新型生物材料可能适用于临床应用。
