Univ. de Granada, Spain.
J Biomed Opt. 2020 May;25(5):1-16. doi: 10.1117/1.JBO.25.5.055002.
Recent generation of bioengineered human skin allowed the efficient treatment of patients with severe skin defects. However, the optical and biomechanical properties of these models are not known.
Three models of bioengineered human skin based on fibrin-agarose biomaterials (acellular, dermal skin substitutes, and complete dermoepidermal skin substitutes) were generated and analyzed.
Optical and biomechanical properties of these artificial human skin substitutes were investigated using the inverse adding-doubling method and tensile tests, respectively.
The analysis of the optical properties revealed that the model that most resembled the optical behavior of the native human skin in terms of absorption and scattering properties was the dermoepidermal human skin substitutes after 7 to 14 days in culture. The time-course evaluation of the biomechanical parameters showed that the dermoepidermal substitutes displayed significant higher values than acellular and dermal skin substitutes for all parameters analyzed and did not differ from the control skin for traction deformation, stress, and strain at fracture break.
We demonstrate the crucial role of the cells from a physical point of view, confirming that a bioengineered dermoepidermal human skin substitute based on fibrin-agarose biomaterials is able to fulfill the minimal requirements for skin transplants for future clinical use at early stages of in vitro development.
新一代生物工程人体皮肤模型使严重皮肤缺陷患者的治疗变得高效。然而，这些模型的光学和生物力学特性尚不清楚。
基于纤维蛋白-琼脂糖生物材料生成并分析三种生物工程人体皮肤模型（非细胞真皮皮肤替代物、完整的真皮表皮皮肤替代物）。
使用反向添加-倍增法和拉伸试验分别对这些人工皮肤替代物的光学和生物力学特性进行了研究。
分析光学特性的结果显示，在培养 7 至 14 天后，吸收和散射特性最接近天然人体皮肤光学行为的模型是真皮表皮人体皮肤替代物。生物力学参数的时程评估表明，与非细胞和真皮皮肤替代物相比，真皮表皮替代物的所有分析参数均显示出显著更高的值，且与对照皮肤相比，在牵引变形、断裂应力和断裂应变方面没有差异。
我们从物理角度证明了细胞的关键作用，证实了基于纤维蛋白-琼脂糖生物材料的生物工程真皮表皮人体皮肤替代物能够满足未来临床应用的早期阶段对皮肤移植物的最低要求。
