Department of Ophthalmology, Firat University School of Medicine, Elazig, Turkey.
Retina. 2012 Feb;32(2):232-41. doi: 10.1097/IAE.0b013e31821e2207.
To investigate the effect of intravitreal tacrolimus on an animal model of proliferative vitreoretinopathy (PVR) and on growth factors implicated in its pathogenesis.
Twenty-one guinea pigs were randomly assigned to one of three groups of seven animals each: no-PVR/saline group (no PVR/intravitreal saline-injected group), PVR/saline group (dispase-induced PVR group, treated with control injections of intravitreal saline), and PVR/tacrolimus group (treatment group, dispase-induced PVR group treated with intravitreal tacrolimus injections). At the end of the experiment, eyes were enucleated and the identification of the stages of PVR was carried out. While a halves of the enucleated globes were evaluated histopathologically for PVR formation, the retinas of the other halves of globes were used for the preparation of retinal homogenates. The transforming growth factor-β, platelet-derived growth factor, and fibroblast growth factor levels in homogenized retina tissues were measured by the enzyme-linked immunosorbent assay method.
When assessing the average PVR stages in terms of severe PVR rates, the PVR/tacrolimus group had significantly improved when compared with the PVR/saline group. The PVR/tacrolimus group demonstrated significantly decreased levels of transforming growth factor-β, platelet-derived growth factor, and fibroblast growth factor when compared with the PVR/saline group and also demonstrated significant improvement in epiretinal membrane formation and retinal fold in the presence of histopathologic levels. The difference in degradation of photoreceptor cells between the PVR/tacrolimus and the PVR/saline groups was not statistically significant.
This study suggests that intravitreal tacrolimus application may suppress PVR development and that tacrolimus may merit investigation for the prophylaxis of PVR.
研究玻璃体内他克莫司对增生性玻璃体视网膜病变（PVR）动物模型及发病机制中相关生长因子的影响。
21 只豚鼠随机分为 3 组，每组 7 只：无 PVR/盐水组（无 PVR/玻璃体内注射生理盐水组）、PVR/盐水组（Dispase 诱导的 PVR 组，玻璃体内注射生理盐水对照治疗）和 PVR/他克莫司组（治疗组，Dispase 诱导的 PVR 组玻璃体内注射他克莫司）。实验结束时，眼球被切除，并对 PVR 分期进行鉴定。一半眼球进行组织病理学评估以观察 PVR 形成情况，另一半眼球的视网膜用于制备视网膜匀浆。通过酶联免疫吸附试验方法测量匀浆视网膜组织中转化生长因子-β、血小板衍生生长因子和碱性成纤维细胞生长因子的水平。
根据严重 PVR 发生率评估平均 PVR 分期时，与 PVR/盐水组相比，PVR/他克莫司组显著改善。与 PVR/盐水组相比，PVR/他克莫司组转化生长因子-β、血小板衍生生长因子和碱性成纤维细胞生长因子水平显著降低，同时在组织病理学水平上也显示出对视网膜内膜形成和视网膜皱襞的显著改善。与 PVR/盐水组相比，PVR/他克莫司组光感受器细胞降解的差异无统计学意义。
本研究表明，玻璃体内应用他克莫司可能抑制 PVR 的发展，他克莫司可能值得进一步研究用于预防 PVR。
