Guoliang Lv, Anye Zhang, Lifu Zhao, Xiaoping Pan, Yimin Zhang, Chengbo Yu, Yuemei Chen, Lanjuan Li
Zhejiang University, Hangzhou, China.
Hepatogastroenterology. 2011 Jul-Aug;58(109):1328-33. doi: 10.5754/hge11034.
BACKGROUND/AIMS: The efficiency of bioartificial liver or cell transplantation for the treatment of liver failure may potentially be diminished by toxic agents accumulated in the patients blood. We investigated the effects of plasma from patients with acute-on-chronic liver failure on the structure and function of immortalized human hepatocytes (HepLL) in vitro.
Plasma was pooled from 8 patients with acute or chronic liver failure. We utilized Transmission Electron Microscopy to observe the ultrastructure of HepLL cells and the activity of proliferation was examined by MTT assay. The percentage of apoptosis cells was measured through Annexin V-FITC apoptosis detection kit. The capacity for detoxification was analyzed using an ammonia elimination assay.
HepLL cells continue to proliferate, even when exposed to plasma derived from patients with 100% liver failure. No significant apoptosis was measured within 48h. The capacity for ammonia elimination was not statistically decreased until 36h incubation with liver failure plasma.
Although the toxic effects of plasma from acute-on-chronic liver failure patients should not be ignored, HepLL cells can maintain their function, when exposed to AoCLF plasma, for at least 24h and could be used as a suitable cell source for a bioartificial liver support system.
背景/目的：生物人工肝或细胞移植治疗肝衰竭的效率可能会因患者血液中积累的毒性物质而降低。我们在体外研究了慢性加急性肝衰竭患者的血浆对永生化人肝细胞（HepLL）结构和功能的影响。
收集了8例急性或慢性肝衰竭患者的血浆。我们利用透射电子显微镜观察HepLL细胞的超微结构，并通过MTT法检测增殖活性。通过Annexin V-FITC凋亡检测试剂盒测量凋亡细胞的百分比。使用氨消除试验分析解毒能力。
即使暴露于来自100%肝衰竭患者的血浆中，HepLL细胞仍继续增殖。48小时内未检测到明显凋亡。与肝衰竭血浆孵育36小时后，氨消除能力才出现统计学上的下降。
尽管慢性加急性肝衰竭患者血浆的毒性作用不容忽视，但HepLL细胞在暴露于慢性加急性肝衰竭血浆时，至少24小时内可维持其功能，可作为生物人工肝支持系统的合适细胞来源。
