Li Jin, Yang Luoyan, Wu Hongtao
Department of Urology, Central Hospital of Xiangtan, Xiangtan Hunan 411100,China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Aug;36(8):754-9. doi: 10.3969/j.issn.1672-7347.2011.08.010.
To observe the proliferation inhibition and apoptosis promotion effect of oridonin on PC-3 cells.
PC-3 cells were treated with different concentrations of oridonin. MTT assay and drug concentration-time survival curve were used to test the effect of oridonin on the PC-3 cells. The percentage of earlier apoptosis cells was analyzed by flow cytometry. The protein expression of caspase-3, Bcl-2, and Bax in the PC-3 cells was detected by Western blot.
Oridonin effectively inhibited the proliferation of PC-3 cells in both concentration- and time-dependent manner, and the IC50 of PC-3 cells was 10.29 μmol/L. HochesT(3)3258 staining and flow cytometry deteced that oridonin induced the apoptosis of PC-3 cells in a concentration-dependent manner (P<0.05). Oridonin down-regulated Bcl-2, up-regulated Bax protein, and activated caspase-3 in a concentration-dependent manner in the PC-3 cells.
The apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.
观察冬凌草甲素对PC-3细胞的增殖抑制及促凋亡作用。
用不同浓度的冬凌草甲素处理PC-3细胞。采用MTT法和药物浓度-时间存活曲线检测冬凌草甲素对PC-3细胞的作用。通过流式细胞术分析早期凋亡细胞的百分比。用蛋白质免疫印迹法检测PC-3细胞中半胱天冬酶-3、Bcl-2和Bax的蛋白表达。
冬凌草甲素能有效抑制PC-3细胞的增殖，呈浓度和时间依赖性，PC-3细胞的半数抑制浓度（IC50）为10.29 μmol/L。HochesT(3)3258染色和流式细胞术检测显示，冬凌草甲素呈浓度依赖性诱导PC-3细胞凋亡（P<0.05）。冬凌草甲素在PC-3细胞中呈浓度依赖性下调Bcl-2、上调Bax蛋白并激活半胱天冬酶-3。
冬凌草甲素诱导PC-3细胞凋亡可能与线粒体途径有关。
