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半乳糖特异性植物凝集素在鉴别诊断积液中腺癌细胞的意义。

Significance of a galactose specific plant lectin for the differential diagnosis of adenocarcinoma cells in effusion.

作者信息

Sujathan K, Jayasree K, Remani P

机构信息

Division of Cytopathology, Regional Cancer Centre, Trivandrum, Kerala, India.

出版信息

J Cytol. 2009 Oct;26(4):134-9. doi: 10.4103/0970-9371.62181.

DOI:10.4103/0970-9371.62181
PMID:21938176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3167997/
Abstract

BACKGROUND

Distinguishing adenocarcinoma cells from reactively proliferated mesothelial cells and macrophages is one of the greatest challenges in the cytodiagnosis of effusions. Aberrant glycosylation of cell surface glycoconjugates is emblematic to malignancy, and lectins being an important class of probes to demonstrate these aberrations, lectin cytochemistry is of great interest to differentiate adenocarcinoma cells from reactive mesothelial cells.

AIM

The present study analyzed the potential of a plant lectin to distinguish malignant cells from reactive mesothelial cells and macrophages.

MATERIALS AND METHODS

Snake gourd lectin (SGL) was isolated, purified and conjugated to horse radish peroxidase (HRP) and incubated with the cells of benign (46) as well as malignant (39) effusions using the standard immunocytochemical method with diaminobenzidine as the chromogen. The lectin-bound areas were quantitatively assessed as mild, moderate and intense binding.

STATISTICAL ANALYSIS

The mean score for benign and malignant effusions were statistically analyzed. Student's 't'-test was performed to assess the significance.

RESULTS

The lectin HRP complex bind to the cytoplasm of benign and malignant cells as well as macrophages. A significantly higher score for intense binding (P = 0.001) was found to differentiate malignant cells from reactive mesothelial cells. Macrophages showed intense irregular binding.

CONCLUSIONS

SGL binding assay can play a role in the differential diagnosis of metastatic adenocarcinoma in effusions.

摘要

背景

在积液的细胞诊断中，区分腺癌细胞与反应性增生的间皮细胞和巨噬细胞是最大的挑战之一。细胞表面糖缀合物的异常糖基化是恶性肿瘤的标志，而凝集素作为一类重要的用于显示这些异常的探针，凝集素细胞化学对于区分腺癌细胞与反应性间皮细胞具有重要意义。

目的

本研究分析了一种植物凝集素区分恶性细胞与反应性间皮细胞和巨噬细胞的潜力。

材料与方法

分离、纯化蛇瓜凝集素（SGL）并将其与辣根过氧化物酶（HRP）偶联，使用以二氨基联苯胺为显色剂的标准免疫细胞化学方法，将其与良性（46例）以及恶性（39例）积液的细胞进行孵育。对凝集素结合区域进行定量评估，分为轻度、中度和重度结合。

统计学分析

对良性和恶性积液的平均得分进行统计学分析。采用学生t检验评估其显著性。

结果

凝集素HRP复合物与良性和恶性细胞以及巨噬细胞的细胞质结合。发现重度结合的得分显著更高（P = 0.001），可用于区分恶性细胞与反应性间皮细胞。巨噬细胞表现出强烈的不规则结合。

结论

SGL结合试验可在积液中转移性腺癌的鉴别诊断中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d53/3167997/d0378b1e5c19/JCytol-26-134-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d53/3167997/99fa8d7cdf04/JCytol-26-134-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d53/3167997/d0378b1e5c19/JCytol-26-134-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d53/3167997/99fa8d7cdf04/JCytol-26-134-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d53/3167997/d0378b1e5c19/JCytol-26-134-g003.jpg

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