基于重组无机焦磷酸酶基因抗原的酶联免疫吸附试验的建立及其对猪肺炎支原体抗体检测的评价。

Development and evaluation of enzyme-linked immunosorbent assay based on recombinant inorganic pyrophosphatase gene antigen for the detection of Mycoplasma suis antibodies.

机构信息

College of Veterinary Medicine and Animal Science, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, China.

出版信息

Res Vet Sci. 2012 Aug;93(1):48-50. doi: 10.1016/j.rvsc.2011.08.016. Epub 2011 Sep 22.

DOI:10.1016/j.rvsc.2011.08.016

PMID:21940024

Abstract

The recombinant ppa protein of Mycoplasma suis migrated to 21 kDa. Using this antigen, an ELISA system to detect the antibody against M. suis infection in swine was established. The rELISA demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with M. suis infection. A comparison of this ELISA system with an indirect hemagglutination assay (IHA) test using 132 swine samples revealed that the positive rate was 34.0% in ELISA and 28.0% in IHA. Compared with IHA, the present rELISA system using recombinant ppa antigen significantly improves the specificity, sensitivity, and stability for serodiagnosis of M. suis infection in swine.

摘要

猪肺炎支原体的重组 ppa 蛋白迁移到 21 kDa。利用该抗原，建立了一种用于检测猪支原体感染抗体的 ELISA 系统。rELISA 在阴性样本中的特异性为 98.5%，在感染支原体的阳性样本中的敏感性为 96.9%。将该 ELISA 系统与间接血凝试验（IHA）比较，使用 132 份猪样本进行比较，结果表明 ELISA 的阳性率为 34.0%，IHA 为 28.0%。与 IHA 相比，本研究使用重组 ppa 抗原的 rELISA 系统显著提高了猪支原体感染血清学诊断的特异性、敏感性和稳定性。