靶向捕获提高了电化学检测细菌病原体的灵敏度。
Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.
机构信息
Department of Urology, The David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4293-6. doi: 10.1128/JCM.01261-11. Epub 2011 Sep 21.
Abstract
We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.
摘要
我们报告了通过使用生物素化的 DNA 靶标特异性捕获（TSC）探针来浓缩和纯化细菌 16S rRNA。对于培养的细菌和来自尿路感染患者的尿液标本，TSC 使 16S rRNA 电化学传感器检测中细菌检测的灵敏度提高了 5-8 倍。
相似文献
1
Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.靶向捕获提高了电化学检测细菌病原体的灵敏度。
J Clin Microbiol. 2011 Dec;49(12):4293-6. doi: 10.1128/JCM.01261-11. Epub 2011 Sep 21.
2
Bacterial detection & identification using electrochemical sensors.使用电化学传感器进行细菌检测与鉴定
J Vis Exp. 2013 Apr 23(74):e4282. doi: 10.3791/4282.
3
Development of an advanced electrochemical DNA biosensor for bacterial pathogen detection.用于细菌病原体检测的先进电化学DNA生物传感器的研发。
J Mol Diagn. 2007 Apr;9(2):158-68. doi: 10.2353/jmoldx.2007.060052.
4
Clinical validation of integrated nucleic acid and protein detection on an electrochemical biosensor array for urinary tract infection diagnosis.电化学生物传感器阵列上的核酸和蛋白质集成检测用于尿路感染诊断的临床验证。
PLoS One. 2011;6(10):e26846. doi: 10.1371/journal.pone.0026846. Epub 2011 Oct 31.
5
Optimal probe length and target location for electrochemical detection of selected uropathogens at ambient temperature.常温下对选定尿路病原体进行电化学检测的最佳探针长度和靶点位置
J Clin Microbiol. 2008 Aug;46(8):2707-16. doi: 10.1128/JCM.00423-08. Epub 2008 Jun 18.
6
A Multiplex Electrochemical Biosensor for Bloodstream Infection Diagnosis.一种用于血流感染诊断的多重电化学生物传感器。
SLAS Technol. 2017 Aug;22(4):466-474. doi: 10.1177/2211068216651232. Epub 2016 May 25.
7
Direct Detection and Identification of Bacterial Pathogens from Urine with Optimized Specimen Processing and Enhanced Testing Algorithm.通过优化样本处理和增强检测算法直接从尿液中检测和鉴定细菌病原体
J Clin Microbiol. 2017 May;55(5):1488-1495. doi: 10.1128/JCM.02549-16. Epub 2017 Mar 1.
8
Rapid, specific and sensitive electrochemical detection of foodborne bacteria.食源细菌的快速、特异性和灵敏的电化学检测
Biosens Bioelectron. 2009 May 15;24(9):2766-71. doi: 10.1016/j.bios.2009.01.042. Epub 2009 Feb 6.
9
Rapid universal identification of bacterial pathogens from clinical cultures by using a novel sloppy molecular beacon melting temperature signature technique.利用新型宽松分子信标熔解温度特征技术快速通用鉴定临床培养物中的细菌病原体。
J Clin Microbiol. 2010 Jan;48(1):258-67. doi: 10.1128/JCM.01725-09. Epub 2009 Nov 18.
10
Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections.广谱 16S rRNA 基因聚合酶链反应在诊断培养阴性细菌感染中的应用。
Clin Infect Dis. 2011 Dec;53(12):1245-51. doi: 10.1093/cid/cir692. Epub 2011 Oct 5.
引用本文的文献
1
Direct-from-specimen microbial growth inhibition spectrums under antibiotic exposure and comparison to conventional antimicrobial susceptibility testing.直接从标本中进行抗生素暴露下的微生物生长抑制谱分析，并与传统的抗菌药物敏感性测试进行比较。
PLoS One. 2022 Feb 16;17(2):e0263868. doi: 10.1371/journal.pone.0263868. eCollection 2022.
2
A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA.一种利用tufA促进对石内光合生物进行调查的宏条形码框架。
BMC Ecol. 2016 Mar 10;16:8. doi: 10.1186/s12898-016-0068-x.
3
Bacterial detection & identification using electrochemical sensors.使用电化学传感器进行细菌检测与鉴定
J Vis Exp. 2013 Apr 23(74):e4282. doi: 10.3791/4282.
本文引用的文献
1
Optimal probe length and target location for electrochemical detection of selected uropathogens at ambient temperature.常温下对选定尿路病原体进行电化学检测的最佳探针长度和靶点位置
J Clin Microbiol. 2008 Aug;46(8):2707-16. doi: 10.1128/JCM.00423-08. Epub 2008 Jun 18.
2
Specific magnetic bead based capture of genomic DNA from clinical samples: application to the detection of group B streptococci in vaginal/anal swabs.基于特异性磁珠从临床样本中捕获基因组DNA：应用于阴道/肛门拭子中B族链球菌的检测
Clin Chem. 2007 Sep;53(9):1570-6. doi: 10.1373/clinchem.2007.091389. Epub 2007 Jul 27.
3
Development of an advanced electrochemical DNA biosensor for bacterial pathogen detection.用于细菌病原体检测的先进电化学DNA生物传感器的研发。
J Mol Diagn. 2007 Apr;9(2):158-68. doi: 10.2353/jmoldx.2007.060052.
4
Magnetic bead-based solid phase for selective extraction of genomic DNA.用于基因组DNA选择性提取的磁珠固相
Anal Biochem. 2006 Aug 15;355(2):285-97. doi: 10.1016/j.ab.2006.05.005. Epub 2006 May 24.
5
APTIMA PCA3 molecular urine test: development of a method to aid in the diagnosis of prostate cancer.安图生物PCA3分子尿液检测：一种辅助前列腺癌诊断方法的研发
Clin Chem. 2006 Jun;52(6):1089-95. doi: 10.1373/clinchem.2005.063289. Epub 2006 Apr 20.
6
Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens.电化学DNA生物传感器在临床尿液标本中快速分子鉴定尿路病原体中的应用。
J Clin Microbiol. 2006 Feb;44(2):561-70. doi: 10.1128/JCM.44.2.561-570.2006.
7
Electrochemical molecular analysis without nucleic acid amplification.无需核酸扩增的电化学分子分析。
Methods. 2005 Sep;37(1):73-83. doi: 10.1016/j.ymeth.2005.05.008. Epub 2005 Oct 4.
8
Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens.用于检测女性尿液和宫颈拭子标本中沙眼衣原体和淋病奈瑟菌的APTIMA Combo 2检测法的性能
J Clin Microbiol. 2003 Jan;41(1):304-9. doi: 10.1128/JCM.41.1.304-309.2003.
9
Guidelines for antimicrobial treatment of uncomplicated acute bacterial cystitis and acute pyelonephritis in women. Infectious Diseases Society of America (IDSA).女性单纯性急性细菌性膀胱炎和急性肾盂肾炎抗菌治疗指南。美国传染病学会（IDSA）。
Clin Infect Dis. 1999 Oct;29(4):745-58. doi: 10.1086/520427.
10
Immunomagnetic cell sorting--pushing the limits.免疫磁珠细胞分选——突破极限
Immunotechnology. 1998 Oct;4(2):89-96. doi: 10.1016/s1380-2933(98)00010-4.
Zotero 插件