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靶向捕获提高了电化学检测细菌病原体的灵敏度。

Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.

机构信息

Department of Urology, The David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.

出版信息

J Clin Microbiol. 2011 Dec;49(12):4293-6. doi: 10.1128/JCM.01261-11. Epub 2011 Sep 21.

DOI:10.1128/JCM.01261-11
PMID:21940468
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3232961/
Abstract

We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.

摘要

我们报告了通过使用生物素化的 DNA 靶标特异性捕获（TSC）探针来浓缩和纯化细菌 16S rRNA。对于培养的细菌和来自尿路感染患者的尿液标本，TSC 使 16S rRNA 电化学传感器检测中细菌检测的灵敏度提高了 5-8 倍。

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本文引用的文献

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J Clin Microbiol. 2008 Aug;46(8):2707-16. doi: 10.1128/JCM.00423-08. Epub 2008 Jun 18.
2
Specific magnetic bead based capture of genomic DNA from clinical samples: application to the detection of group B streptococci in vaginal/anal swabs.基于特异性磁珠从临床样本中捕获基因组DNA：应用于阴道/肛门拭子中B族链球菌的检测
Clin Chem. 2007 Sep;53(9):1570-6. doi: 10.1373/clinchem.2007.091389. Epub 2007 Jul 27.
3
Development of an advanced electrochemical DNA biosensor for bacterial pathogen detection.用于细菌病原体检测的先进电化学DNA生物传感器的研发。
J Mol Diagn. 2007 Apr;9(2):158-68. doi: 10.2353/jmoldx.2007.060052.
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Magnetic bead-based solid phase for selective extraction of genomic DNA.用于基因组DNA选择性提取的磁珠固相
Anal Biochem. 2006 Aug 15;355(2):285-97. doi: 10.1016/j.ab.2006.05.005. Epub 2006 May 24.
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APTIMA PCA3 molecular urine test: development of a method to aid in the diagnosis of prostate cancer.安图生物PCA3分子尿液检测：一种辅助前列腺癌诊断方法的研发
Clin Chem. 2006 Jun;52(6):1089-95. doi: 10.1373/clinchem.2005.063289. Epub 2006 Apr 20.
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Use of electrochemical DNA biosensors for rapid molecular identification of uropathogens in clinical urine specimens.电化学DNA生物传感器在临床尿液标本中快速分子鉴定尿路病原体中的应用。
J Clin Microbiol. 2006 Feb;44(2):561-70. doi: 10.1128/JCM.44.2.561-570.2006.
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Electrochemical molecular analysis without nucleic acid amplification.无需核酸扩增的电化学分子分析。
Methods. 2005 Sep;37(1):73-83. doi: 10.1016/j.ymeth.2005.05.008. Epub 2005 Oct 4.
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Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens.用于检测女性尿液和宫颈拭子标本中沙眼衣原体和淋病奈瑟菌的APTIMA Combo 2检测法的性能
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