Iwai N, Inagami T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee.
Kidney Int. 1990 Jun;37(6):1460-5. doi: 10.1038/ki.1990.136.
Using the technique of differential plaque filter hybridization, a rat cDNA was isolated whose corresponding gene expression in the kidney was positively modulated up to threefold by sodium depletion. This mRNA was more abundantly expressed in the kidneys of 17-week-old spontaneously hypertensive rats than those of age-matched Wistar-Kyoto rats. The putative protein encoded by this cDNA is a homologue of cyclophilin, a cytosolic binding protein for cyclosporin A. This cyclophilin-like protein mRNA was expressed in all the tissues examined, including the adrenal, atrium, brain, kidney, liver, lung, spleen, and ventricle. Sodium depletion in rats increased the expression level of this mRNA not only in the kidney but also in the liver. The administration of cyclosporin A in rats increased the expression level of this mRNA in the kidneys and livers. By virtue of its possible involvement in sodium homeostasis and its homology to cyclophilin, this molecule might have significant implications in the mechanism of cyclosporine-induced renal insufficiency and hypertension.
运用差异噬菌斑滤膜杂交技术，分离出一个大鼠cDNA，其在肾脏中的相应基因表达在钠耗竭时可正向调节至三倍。该mRNA在17周龄自发性高血压大鼠肾脏中的表达量比年龄匹配的Wistar-Kyoto大鼠肾脏中的表达量更丰富。此cDNA编码的推定蛋白是亲环蛋白的同源物，亲环蛋白是环孢菌素A的一种胞质结合蛋白。这种亲环蛋白样蛋白mRNA在所检测的所有组织中均有表达，包括肾上腺、心房、脑、肾、肝、肺、脾和心室。大鼠钠耗竭不仅增加了该mRNA在肾脏中的表达水平，还增加了其在肝脏中的表达水平。给大鼠注射环孢菌素A会增加该mRNA在肾脏和肝脏中的表达水平。鉴于其可能参与钠稳态以及与亲环蛋白的同源性，该分子可能在环孢素诱导的肾功能不全和高血压机制中具有重要意义。
