Zhu Lan, Feng Li, Sun Ying, Wang Xian-Hua, Ma Xiao-Bing
Department of Pathology, Hebei United University, Tangshan, Hebei province 063000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 Apr;29(4):246-50.
Effects of silicotic alveolar macrophages exposed to SiO2 on the expression of type III collagen and type
To study the effects of supernatant of alveolar macrophages (AM) exposed to SiO2 on the expression of type III collagen and type III procollagen in human lung fibroblasts (HELF) and the intervention effects of anti-TGF-beta1 antibody.
AMs collected from a silicotic by bronchoalveolar lavage were divided into 2 parts, one part was exposed to SiO2 and other part served as control. The supernatant was obtained from AMs cultured for 18 h. HELF were divided into (1) exposure group, which was added with supernatant from AMs exposed to SiO2; (2) control group, which was added with the supernatant from AMs not exposed to SiO2; (3) blank control group, which was added with DMEM; (4) exposure group plus anti-TGF-beta1 antibody (10 microg/ml); (5) control group plus anti-TGF-beta1 antibody (10 microg/ml). (1)-(3) groups were cultured for 6, 12, 18, 24, 36, 48h, respectively. (4)-(5) groups were cultured for 18, 24, 36, respectively. Immunocytochemical test and Western blot assay were used to detect pC III expression levels in HELF and C III expression levels in the supernatant of HELF culture, respectively.
The pC III expression levels of exposure group were 0.1423 +/- 0.0107, 0.1624 +/- 0.0011, 0.1925 +/- 0.0050, 0.2421 +/- 0.0097 and 0.2103 +/- 0.0103, respectively, which were significantly higher than those (0.1212 +/- 0.0079, 0.1414 +/- 0.0058, 0.1620 +/- 0.0081, 0.1965 +/- 0.0103, 0.1715 +/- 0.0116) of control group (P < 0.05 or P < 0.01). The C III levels of exposure group were (0.2559 +/- 0.0061, 0.3249 +/- 0.0110, 0.4171 +/- 0.0193, 0.5441 +/- 0.0452, 0.4751 +/- 0.0252), respectively, which were significantly higher than control group (0.2296 +/- 0.0121, 0.2778 +/- 0.0116, 0.3367 +/- 0.0269, 0.3722 +/- 0.0214). The pC III and C III expression levels of exposure plus anti-TGF-beta1 antibody group were significantly lower than those of control plus anti-TGF-beta1 antibody group (P < 0.05 or P < 0.01).
AMs exposed to SiO2 can induce the elevated pC IIII and C III expression levels in HELF by TGFbetaP1 to some extent.
暴露于二氧化硅的矽肺肺泡巨噬细胞对III型胶原蛋白表达的影响
研究暴露于二氧化硅的肺泡巨噬细胞（AM）上清液对人肺成纤维细胞（HELF）中III型胶原蛋白和III型前胶原蛋白表达的影响以及抗转化生长因子-β1抗体的干预作用。
通过支气管肺泡灌洗从矽肺患者收集的AMs分为两部分，一部分暴露于二氧化硅，另一部分作为对照。将培养18小时的AMs的上清液取出。HELF分为：（1）暴露组，加入暴露于二氧化硅的AMs的上清液；（2）对照组，加入未暴露于二氧化硅的AMs的上清液；（3）空白对照组，加入DMEM；（4）暴露组加抗转化生长因子-β1抗体（10微克/毫升）；（5）对照组加抗转化生长因子-β1抗体（10微克/毫升）。（1）-（3）组分别培养6、12、18、24、36、48小时。（4）-（5）组分别培养18、24、36小时。分别采用免疫细胞化学检测和蛋白质印迹法检测HELF中前C III表达水平和HELF培养上清液中C III表达水平。
暴露组的前C III表达水平分别为0.1423±0.0107、0.1624±0.0011、0.1925±0.0050、0.2421±0.0097和0.2103±0.0103，显著高于对照组（0.1212±0.0079、0.1414±0.0058、0.1620±0.0081、0.1965±0.0103、0.1715±0.0116）（P<0.05或P<0.01）。暴露组的C III水平分别为（0.2559±0.0061、0.3249±0.0110、0.4171±0.0193、0.5441±0.0452、0.4751±0.0252），显著高于对照组（0.2296±0.0121、0.2778±0.0116、0.3367±0.0269、0.3722±0.0214）。暴露加抗转化生长因子-β1抗体组的前C III和C III表达水平显著低于对照加抗转化生长因子-β1抗体组（P<0.05或P<0.01）。
暴露于二氧化硅的AMs可通过转化生长因子-β1在一定程度上诱导HELF中前C III和C III表达水平升高。
