School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
Langmuir. 2011 Nov 1;27(21):12782-7. doi: 10.1021/la203124e. Epub 2011 Sep 29.
The enhancement of weak Raman signals has been challenged to obtain high-quality signals of surface-enhanced Raman scattering (SERS). By employing the Parkinson's disease-related protein of α-synuclein, we introduce SERS-active gold nanoparticles (AuNPs) individually isolated with an ultrathin α-synuclein shell and their 2-D array into a tightly packed monolayer on a glass support, which permits a quantitative SERS measurement of phthalocyanine tetrasulfonate (PcTS), a chemical ligand of the pathological protein. Subsequently, the PcTS-bound SERS substrate was also shown to be capable of discriminating two biologically important metal ions of iron and copper by detecting copper ion to the sub-ppm level in a highly selective manner via the in situ chemical reaction of metal chelation to PcTS. The strategy of using the protein-based 2-D AuNP SERS platform, therefore, could be further developed into a custom-made protein-based biosensor system for the detection of not only specific chemical/biological ligands of the immobilized coat proteins but also their biochemical reactivities.
弱 Raman 信号的增强一直是获得表面增强拉曼散射（SERS）高质量信号的挑战。通过使用帕金森病相关的α-突触核蛋白，我们引入了具有超薄α-突触核蛋白壳的 SERS 活性金纳米颗粒（AuNP），并将其二维阵列单独隔离到玻璃基底上的紧密堆积单层中，这允许对病理蛋白的化学配体对苯二甲酸四磺酸钠（PcTS）进行定量 SERS 测量。随后，通过 PcTS 与金属离子的原位螯合反应，PcTS 结合的 SERS 基底还表现出能够以高度选择性的方式区分两种重要的生物金属离子铁和铜，检测铜离子的浓度低至亚ppm 级。因此，使用基于蛋白质的二维 AuNP SERS 平台的策略可以进一步开发成定制的基于蛋白质的生物传感器系统，不仅可以检测固定涂层蛋白的特定化学/生物配体，还可以检测其生化反应性。
