Winder Catherine L, Lanthaler Karin
Manchester Centre for Integrative Systems Biology and Department of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, United Kingdom.
Methods Enzymol. 2011;500:261-75. doi: 10.1016/B978-0-12-385118-5.00014-1.
When acquiring data for systems biology studies, it is essential to perform the experiments in controlled and reproducible conditions. Advances in the fields of proteomics and metabolomics allow the quantitative analysis of the components of the biological cell. It is essential to include a method in the experimental pipeline to culture the biological system in controlled and reproducible conditions to facilitate the acquisition of high-quality data. The employment of continuous culture methods for the growth of microorganisms is an ideal tool to achieve these objectives. This chapter will review the continuous culture approaches which may be applied in such studies, outline the experimental options which should be considered, and describe the approach applied in the production of steady-state cultures of Saccharomyces cerevisiae.
在为系统生物学研究获取数据时，在可控且可重复的条件下进行实验至关重要。蛋白质组学和代谢组学领域的进展使得对生物细胞成分进行定量分析成为可能。在实验流程中纳入一种方法，以便在可控且可重复的条件下培养生物系统，对于获取高质量数据非常关键。采用连续培养方法来培养微生物是实现这些目标的理想工具。本章将回顾可应用于此类研究的连续培养方法，概述应考虑的实验选项，并描述在酿酒酵母稳态培养物生产中应用的方法。
