一个编码棉花异柠檬酸裂解酶完整氨基酸序列的cDNA克隆的特性分析。
Characterization of a cDNA clone encoding the complete amino acid sequence of cotton isocitrate lyase.
作者信息
Turley R B, Choe S M, Trelease R N
机构信息
Department of Botany, Arizona State University, Tempe 85287-1601.
出版信息
Biochim Biophys Acta. 1990 Jun 21;1049(2):223-6. doi: 10.1016/0167-4781(90)90045-4.
A cDNA clone encoding the glyoxysomal enzyme isocitrate lyase (ICL) (EC 4.1.3.1) was isolated from a library prepared from cotton (Gossypium hirsutum L.) cotyledon poly(A)+ RNA. The clone is 1893 basepairs (bp) in length and contains a 1728 bp open reading frame encoding a polypeptide of 576 residues (Mr = 64,741). The deduced amino acid sequence of cotton ICL is 85.2%, 90.3% and 41.1% identical to ICL from rapeseed, castor bean and E. coli, respectively. Cotton ICL has a C-terminal tripeptide of A-R-M which is a putative trafficking signal for peroxisome (glyoxysome) proteins.
从由棉花（陆地棉）子叶多聚腺苷酸加尾RNA构建的文库中分离出了一个编码乙醛酸循环体酶异柠檬酸裂解酶（ICL）（EC 4.1.3.1）的cDNA克隆。该克隆长度为1893个碱基对（bp），包含一个1728 bp的开放阅读框，编码一个由576个氨基酸残基组成的多肽（Mr = 64,741）。棉花ICL推导的氨基酸序列与油菜籽、蓖麻和大肠杆菌的ICL分别有85.2%、90.3%和41.1%的同源性。棉花ICL有一个A-R-M的C端三肽，这是过氧化物酶体（乙醛酸循环体）蛋白的一个假定转运信号。
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