Department of Chemical Engineering, École Polytechnique de Montréal, Montréal, Québec, Canada H3C 3A7.
Anal Biochem. 2011 Dec 15;419(2):140-4. doi: 10.1016/j.ab.2011.08.051. Epub 2011 Sep 8.
Surface plasmon resonance-based biosensors have been applied to the determination of macromolecule concentration. Up to now, the proposed experimental approaches have relied either on the generation of a calibration curve that exploits only a few data points from each sensorgram or on multiple injections of the unknown sample at various flow rates. In this article, we show that prior knowledge of the kinetic parameters related to the interaction of the species with a given partner could advantageously reduce the number of injections required by both aforementioned methods, thereby reducing experimental time while maintaining a good level of confidence on the determined concentrations.
基于表面等离子体共振的生物传感器已被应用于大分子浓度的测定。到目前为止，所提出的实验方法要么依赖于生成校准曲线，该曲线仅利用每个传感器图中的几个数据点，要么依赖于以不同流速对未知样品进行多次注入。在本文中，我们表明，与物种与给定配体相互作用相关的动力学参数的先验知识可以有利地减少上述两种方法所需的注入次数，从而在保持对确定浓度的良好置信度的同时减少实验时间。
