The effect of prior surgical disconnection of the anterior mediobasal hypothalamus on LHRH release in vitro.

The degenerative responses of luteinizing hormone-releasing hormone (LHRH)-containing neurons within the mediobasal hypothalamus (MBH) after knife cut lesions (FC) made in the frontal plane of the retrochiasmatic hypothalamus include a reduced number of LHRH-immunoreactive (ir) nerve terminals in the median eminence, reduction in LHRH content of the MBH and growth of novel irLHRH-containing neural processes into FC scar tissue. We have now investigated basal and secretogogue-evoked LHRH release in vitro from the preoptic area-MBH (POA-MBH) of adult male rats at 10 or 60 days after FC. Basal LHRH release rate (P less than 0.05) and total (P less than 0.01) amount released 60 days after FC were reduced when compared to control (CONT) hypothalami, but not shams. A 30 min pulse of naloxone (NAL, 1 mg/ml) stimulated greater than 2-fold relative increase in LHRH release for all groups; however, the total amount of LHRH released by FC hypothalami was less (P less than 0.05) than that of CONT, but not sham POA-MBHs. Although exposure to elevated KCl significantly increased (P less than 0.01) LHRH release for all 3 groups, the FC secretory response was less than that of both CONT (P less than 0.05) and sham (P less than 0.01) groups. In the second experiment single POA-MBH were perifused at 10 days (sham and FC) or 60 days (CONT, sham and FC) after surgery.(ABSTRACT TRUNCATED AT 250 WORDS)