de Vin Filip, De Maeyer Joris H, Lefebvre Romain A
Heymans Institute of Pharmacology, Ghent University, Ghent, Belgium.
Neuroreport. 2011 Dec 7;22(17):892-6. doi: 10.1097/WNR.0b013e32834c7fd4.
5-HT₄ receptor (5-HT₄R) activation induces procognitive effects. This might be related to stimulation of hippocampal acetylcholine release, which has been shown for 5-HT₄R agonists in in-vivo models. We investigated the influence of the 5-HT₄R agonists, prucalopride and BIMU-8, on acetylcholine release in rat hippocampal brain slices. In contrast to the report by Siniscalchi et al., no facilitating effect of 5-HT₄R agonists on electrically evoked acetylcholine could be shown. The in our hands absence of an effect by 5-HT₄R agonists illustrates that an in-vitro evaluation of 5-HT₄R agonists on hippocampal acetylcholine release is not a straightforward model to study the relationship between hippocampal 5-HT₄Rs and hippocampal acetylcholine release.
5-羟色胺4型受体（5-HT₄R）激活可诱导认知促进作用。这可能与海马乙酰胆碱释放的刺激有关，在体内模型中，5-HT₄R激动剂已显示出这种作用。我们研究了5-HT₄R激动剂普芦卡必利和BIMU-8对大鼠海马脑片乙酰胆碱释放的影响。与西尼斯卡尔基等人的报告相反，未显示5-HT₄R激动剂对电诱发乙酰胆碱有促进作用。在我们的实验中，5-HT₄R激动剂无作用表明，对5-HT₄R激动剂对海马乙酰胆碱释放进行体外评估，并非是研究海马5-HT₄R与海马乙酰胆碱释放之间关系的直接模型。
