Doanh Pham Ngoc, Dung Do Trung, Thach Dang Thi Cam, Horii Yoichiro, Shinohara Akio, Nawa Yukifumi
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Viet Nam.
Parasitol Int. 2011 Dec;60(4):534-7. doi: 10.1016/j.parint.2011.09.001. Epub 2011 Sep 17.
Parasitological and sero-epidemiological surveys for human paragonimiasis were conducted in three provinces of Viet Nam. A total of 590 participants from two known endemic areas of human paragonimiasis (Sinho district of Laichau province and Lucyen district of Yenbai province) and from Dakrong district of Quangtri province where we recently found crab hosts heavily infected with Paragonimus westermani metacercariae. By multiple dot-ELISA screening, 28 (12.7%) out of 220 participants in Sinho district of Laichau province and 4 (3.3%) out of 120 participants in Lucyen district of Yenbai province were proven to be antibody-positive against the Paragonimus antigen. None of the 250 sera of the residents in Dakrong, Quangtri province, gave sero-positivity. Among a total of 32 sero-positive patients Paragonimus eggs were found in 6 cases. ITS2 sequences were successfully determined from a single Paragonimus egg from each patient. The results of homology search by BLAST and alignment clearly confirmed that Paragonimus eggs collected from 6 patients were all of Paragonimus heterotremus. The pathogenicity of P. westermani for human paragonimiasis in Viet Nam is still questionable and needs to be explored in the future.
在越南的三个省份开展了人体肺吸虫病的寄生虫学和血清流行病学调查。共有590名参与者，分别来自人体肺吸虫病的两个已知流行区（莱州省新和区以及宣光省禄劝区），还有来自广治省德容区，我们最近在该地区发现蟹宿主感染卫氏并殖吸虫囊蚴的情况严重。通过多次斑点酶联免疫吸附试验筛查，莱州省新和区220名参与者中有28人（12.7%）、宣光省禄劝区120名参与者中有4人（3.3%）被证明对肺吸虫抗原呈抗体阳性反应。广治省德容区居民的250份血清样本均未出现血清阳性反应。在总共32名血清阳性患者中，有6例发现了肺吸虫卵。成功从每位患者的单个肺吸虫卵中测定了ITS2序列。通过BLAST进行的同源性搜索和比对结果清楚地证实，从6名患者采集的肺吸虫卵均为异盘并殖吸虫。卫氏并殖吸虫对越南人体肺吸虫病的致病性仍存在疑问，未来需要进一步探究。
