Department of Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Oncol Rep. 2012 Jan;27(1):246-9. doi: 10.3892/or.2011.1469. Epub 2011 Sep 22.
Thymidylate synthase (TS) gene contains 28-bp polymorphic sequence and 6-bp insert at the 5'- and 3'-untranslated region, respectively. We investigated the role of these two polymorphic traits on TS mRNA expression in nine different cultured human cancer cell lines in vitro. Three cell lines each were 2R/2R, 2R/3R and 3R/3R genotypes. Six of the nine cell lines tested homozygous for the presence of 6-bp insert (+6/+6) and the rest three lacked this insert (-6/-6). TS expression analyses associated homozygous three repeats (3R/3R) to higher TS expression.
胸苷酸合成酶 (TS) 基因含有 28 个碱基对的多态性序列和 6 个碱基对的插入，分别位于 5'和 3'非翻译区。我们在体外研究了这两种多态性特征对 9 种不同培养的人类癌细胞系中 TS mRNA 表达的作用。三种细胞系的基因型分别为 2R/2R、2R/3R 和 3R/3R。在 9 种测试的细胞系中，有 6 种为 6 个碱基对插入（+6/+6）的纯合子，其余 3 种则缺乏该插入（-6/-6）。TS 表达分析表明，纯合三个重复（3R/3R）与更高的 TS 表达相关。
