Chidgey M A, Harrison T M
Department of Biochemistry, University of Leicester, England.
Eur J Biochem. 1990 May 31;190(1):139-44. doi: 10.1111/j.1432-1033.1990.tb15556.x.
The rat pheochromocytoma cell line PC12 targets secretory proteins into two distinct pathways. When DNA encoding human prorenin was transfected into PC12 cells, the protein was sorted into the regulated secretory pathway and released with similar kinetics to noradrenaline upon carbachol stimulation. To determine whether information for targeting prorenin lies within the pro-peptide we have transfected PC12 cells with a construct lacking the pro-peptide coding sequence. The transformed line secretes an apparently fully active enzyme and responds to carbachol stimulation with a rapid release of renin activity. We conclude that the pro-peptide of renin is not essential for targeting the protein to the regulated pathway in PC12 cells.
大鼠嗜铬细胞瘤细胞系PC12将分泌蛋白导向两条不同的途径。当将编码人肾素原的DNA转染到PC12细胞中时，该蛋白被分选到调节性分泌途径中，并在卡巴胆碱刺激下以与去甲肾上腺素相似的动力学释放。为了确定肾素原靶向的信息是否存在于前肽中，我们用一个缺乏前肽编码序列的构建体转染了PC12细胞。转化后的细胞系分泌一种显然完全有活性的酶，并在卡巴胆碱刺激下快速释放肾素活性。我们得出结论，肾素的前肽对于将该蛋白靶向PC12细胞中的调节途径不是必需的。
