Phoenix D A, Pratt J M
Department of Biochemistry, University of Liverpool, England.
Eur J Biochem. 1990 Jun 20;190(2):365-9. doi: 10.1111/j.1432-1033.1990.tb15584.x.
By treating vesicles prepared from Escherichia coli K12 with various reagents, we have investigated the mechanism by which penicillin-binding protein 5 anchors to the inner membrane. The results indicate that there are two forms of anchoring; one which is inaccessible to urea and probably inserted into the bilayer and one which is accessible. Association of the accessible form with the membrane seems to involve significant hydrophobic interaction and this form is triggered to undergo reversible 'insertion' by a decrease in pH.
