地钱（Marchantia polymorpha）叶绿体tRNA（Lys）基因突变体在大肠杆菌中的功能表达。
Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.
作者信息
Nakahigashi K, Inokuchi H, Ozeki H
机构信息
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
出版信息
FEBS Lett. 1990 Jun 4;265(1-2):59-62. doi: 10.1016/0014-5793(90)80883-k.
PMID:2194832
Abstract
The anticodon of the tRNA(Lys) gene (trnK) in the liverwort, Marchantia polymorpha, was artificially converted to an amber anticodon. This mutant tRNA(Lys) (CTA) gene carrying either the intron of the C27-C43 mismatch at the anticodon-stem is not functional in Escherichia coli, but without both of them, it does work as a tRNA(Lys) amber suppressor.
摘要
在苔类植物多歧苏铁（Marchantia polymorpha）中，tRNA（Lys）基因（trnK）的反密码子被人工转化为琥珀色反密码子。这种携带反密码子茎上C27 - C43错配内含子的突变tRNA（Lys）（CTA）基因在大肠杆菌中无功能，但没有这两个内含子时，它可作为tRNA（Lys）琥珀色抑制子发挥作用。
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