Lin Yen-Hsiu, Tseng Wei-Lung
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
Methods Mol Biol. 2011;790:167-72. doi: 10.1007/978-1-61779-319-6_12.
A sample preparation method to detect small molecules in laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) was developed using bare gold nanoparticles (AuNPs) as matrices. In this sample preparation method, the analyte is deposited first and then followed by the bare AuNPs. Neutral steroids and carbohydrates, which are difficult to ionize, using organic matrices, are cationized efficiently by combining AuNP-assisted LDI-TOF MS with this sample preparation method. As compared to the dried-droplet method (i.e., analyte and bare AuNPs are mixed and dried together), this method offers distinct advantages for improving shot-to-shot reproducibility, increasing the ionization efficiency of the analyte, and reducing sample preparation time.
开发了一种以裸金纳米颗粒（AuNPs）为基质的用于激光解吸/电离飞行时间质谱（LDI-TOF MS）中检测小分子的样品制备方法。在这种样品制备方法中，先沉积分析物，然后再加入裸AuNPs。通过将AuNP辅助的LDI-TOF MS与这种样品制备方法相结合，难以用有机基质电离的中性类固醇和碳水化合物能够有效地阳离子化。与干滴法（即将分析物和裸AuNPs混合并一起干燥）相比，该方法在提高逐次进样重现性、提高分析物的电离效率以及减少样品制备时间方面具有明显优势。
