膦酸锆-磁性纳米颗粒对磷酸化肽段的选择性捕获

Selective capture of phosphopeptides by zirconium phosphonate-magnetic nanoparticles.

作者信息

Zhao Liang, Wu Ren'an, Zou Hanfa

机构信息

National Chromatographic R & A Center, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

出版信息

Methods Mol Biol. 2011;790:215-22. doi: 10.1007/978-1-61779-319-6_17.

DOI:10.1007/978-1-61779-319-6_17

PMID:21948418

Abstract

Immobilized metal affinity chromatography is a widely used method for the enrichment of phosphopeptides from proteolytic digests prior to mass spectrometric analysis. Here, we describe the selective enrichment of phosphopeptides from tryptic digests of proteins (α- and β-caseins) by zirconium phosphonate-magnetic Fe(3)O(4)/SiO(2) (core/shell) nanoparticles for phosphoproteome analysis with MALDI-TOF mass spectrometry.

摘要

固定化金属亲和色谱法是一种广泛应用的方法，用于在质谱分析之前从蛋白水解消化物中富集磷酸化肽段。在此，我们描述了通过膦酸锆-磁性Fe(3)O(4)/SiO(2)（核/壳）纳米颗粒从蛋白质（α-和β-酪蛋白）的胰蛋白酶消化物中选择性富集磷酸化肽段，用于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的磷酸化蛋白质组分析。