合成生命的十大重大挑战。
The ten grand challenges of synthetic life.
作者信息
Porcar Manuel, Danchin Antoine, de Lorenzo Victor, Dos Santos Vitor A, Krasnogor Natalio, Rasmussen Steen, Moya Andrés
出版信息
Syst Synth Biol. 2011 Jun;5(1-2):1-9. doi: 10.1007/s11693-011-9084-5. Epub 2011 Aug 5.
Abstract
The construction of artificial life is one of the main scientific challenges of the Synthetic Biology era. Advances in DNA synthesis and a better understanding of regulatory processes make the goal of constructing the first artificial cell a realistic possibility. This would be both a fundamental scientific milestone and a starting point of a vast range of applications, from biofuel production to drug design. However, several major issues might hamper the objective of achieving an artificial cell. From the bottom-up to the selection-based strategies, this work encompasses the ten grand challenges synthetic biologists will have to be aware of in order to cope with the task of creating life in the lab.
摘要
人工生命的构建是合成生物学时代的主要科学挑战之一。DNA合成技术的进步以及对调控过程的更深入理解，使得构建首个人工细胞的目标成为现实可能。这将既是一个具有里程碑意义的基础科学成就，也是从生物燃料生产到药物设计等广泛应用的起点。然而，几个主要问题可能会阻碍实现人工细胞这一目标。从自下而上的策略到基于选择的策略，这项工作涵盖了合成生物学家为应对在实验室创造生命这一任务而必须了解的十大重大挑战。
相似文献
1
The ten grand challenges of synthetic life.
Syst Synth Biol. 2011 Jun;5(1-2):1-9. doi: 10.1007/s11693-011-9084-5. Epub 2011 Aug 5.
2
Division in synthetic cells.
Chem Soc Rev. 2023 May 22;52(10):3307-3325. doi: 10.1039/d2cs00985d.
3
[Advances in artificial cells based on microfluidic chips].
Sheng Wu Gong Cheng Xue Bao. 2024 Jul 25;40(7):2100-2119. doi: 10.13345/j.cjb.240086.
4
Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology.
Biochem Soc Trans. 2016 Jun 15;44(3):723-30. doi: 10.1042/BST20160052.
5
Compartmentalized Cell-Free Expression Systems for Building Synthetic Cells.
Adv Biochem Eng Biotechnol. 2023;186:77-101. doi: 10.1007/10_2023_221.
6
Artificial Cells: Synthetic Compartments with Life-like Functionality and Adaptivity.
Acc Chem Res. 2017 Apr 18;50(4):769-777. doi: 10.1021/acs.accounts.6b00512. Epub 2017 Jan 17.
7
Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology.
Angew Chem Int Ed Engl. 2021 Mar 8;60(11):5602-5611. doi: 10.1002/anie.202006941. Epub 2020 Oct 13.
8
Compartmentalizing Cell-Free Systems: Toward Creating Life-Like Artificial Cells and Beyond.
ACS Synth Biol. 2020 Nov 20;9(11):2881-2901. doi: 10.1021/acssynbio.0c00433. Epub 2020 Oct 23.
9
Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology.
Angew Chem Weinheim Bergstr Ger. 2021 Mar 8;133(11):5662-5671. doi: 10.1002/ange.202006941. Epub 2020 Oct 13.
10
Macromolecular crowding: chemistry and physics meet biology (Ascona, Switzerland, 10-14 June 2012).
Phys Biol. 2013 Aug;10(4):040301. doi: 10.1088/1478-3975/10/4/040301. Epub 2013 Aug 2.
引用本文的文献
1
On the Response of Proteinoid Ensembles to Fibonacci Sequences.
ACS Omega. 2025 Mar 5;10(10):10401-10424. doi: 10.1021/acsomega.4c10571. eCollection 2025 Mar 18.
2
Empirical vitalism: observing an organism's formative power within an active and co-constitutive relation between subject and object.
Hist Philos Life Sci. 2025 Jan 24;47(1):9. doi: 10.1007/s40656-024-00649-z.
3
Molecular and cellular level characterization of cytoskeletal mechanics using a quartz crystal microbalance.
Cytoskeleton (Hoboken). 2023 May-Jun;80(5-6):100-111. doi: 10.1002/cm.21752. Epub 2023 Mar 24.
4
Artificial cell design: reconstructing biology for life science applications.
Emerg Top Life Sci. 2022 Dec 22;6(6):619-627. doi: 10.1042/ETLS20220050.
5
Building a community to engineer synthetic cells and organelles from the bottom-up.
Elife. 2021 Dec 20;10:e73556. doi: 10.7554/eLife.73556.
6
On an algorithmic definition for the components of the minimal cell.
PLoS One. 2018 Jun 1;13(6):e0198222. doi: 10.1371/journal.pone.0198222. eCollection 2018.
7
Scientific iconoclasm and active imagination: synthetic cells as techno-scientific mandalas.
Life Sci Soc Policy. 2018 May 14;14(1):10. doi: 10.1186/s40504-018-0075-0.
8
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.
Nat Commun. 2018 Apr 20;9(1):1583. doi: 10.1038/s41467-018-03926-1.
9
Bacterial cell-free expression technology to systems engineering and optimization.
Synth Syst Biotechnol. 2017 Aug 7;2(2):97-104. doi: 10.1016/j.synbio.2017.07.004. eCollection 2017 Jun.
10
Image of Synthetic Biology and Nanotechnology: A Survey among University Students.
Front Genet. 2017 Sep 20;8:122. doi: 10.3389/fgene.2017.00122. eCollection 2017.
本文引用的文献
1
Beware of metaphors: chasses and orthogonality in synthetic biology.
Bioeng Bugs. 2011 Jan-Feb;2(1):3-7. doi: 10.4161/bbug.2.1.13388.
2
Sugar synthesis in a protocellular model leads to a cell signalling response in bacteria.
Nat Chem. 2009 Aug;1(5):377-83. doi: 10.1038/nchem.296. Epub 2009 Jul 24.
3
Interactions between catalysts and amphiphilic structures and their implications for a protocell model.
Chemphyschem. 2011 Mar 14;12(4):828-35. doi: 10.1002/cphc.201000843. Epub 2011 Feb 23.
4
Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'.
Nature. 2011 Jan 13;469(7329):212-5. doi: 10.1038/nature09565. Epub 2010 Dec 8.
5
Distributed biological computation with multicellular engineered networks.
Nature. 2011 Jan 13;469(7329):207-11. doi: 10.1038/nature09679. Epub 2010 Dec 8.
6
Environmental biosafety in the age of synthetic biology: do we really need a radical new approach? Environmental fates of microorganisms bearing synthetic genomes could be predicted from previous data on traditionally engineered bacteria for in situ bioremediation.
Bioessays. 2010 Nov;32(11):926-31. doi: 10.1002/bies.201000099. Epub 2010 Oct 8.
7
Detection of association and fusion of giant vesicles using a fluorescence-activated cell sorter.
Langmuir. 2010 Oct 5;26(19):15098-103. doi: 10.1021/la102689v.
8
Life after the synthetic cell.
Nature. 2010 May 27;465(7297):422-4. doi: 10.1038/465422a.
9
Creation of a bacterial cell controlled by a chemically synthesized genome.
Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20.
10
Stochastic and deterministic multiscale models for systems biology: an auxin-transport case study.
BMC Syst Biol. 2010 Mar 26;4:34. doi: 10.1186/1752-0509-4-34.
Zotero 插件