Department of Emergency, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Lymphology. 2011 Jun;44(2):82-8.
Difficulty in collecting lymph samples in small animals has impeded studies on lymphatic function and lymph composition. Here we report a simple and effective modified rat model for thoracic duct lymph drainage where animals remain in full consciousness and have free movement and access to water and food over 12 hours. The operative procedure required approximately 30 minutes to perform. Mean lymph drainage was 0.71 +/- 0.33 ml/h, and protein concentration did not change significantly (mean 37 +/- 2.59 mg/ml) over the 12 hours. However, the number of lymphocytes fluctuated widely between 0.08 +/- 0.03 x 10(6)/ml and 12.17 +/- 6.58 x 10(6)/ml. This modified animal model of thoracic duct lymph collection avoids influences of lipid intake, general anesthesia, or limited activity of animals on experimental outcomes, and therefore more accurately reflects lymph flow and composition under normal physiological conditions.
在小动物中收集淋巴样本的困难阻碍了对淋巴功能和淋巴成分的研究。在这里，我们报告了一种简单有效的改良大鼠胸导管淋巴引流模型，该模型中动物保持完全清醒，并可自由移动，12 小时内可自由饮水和进食。手术过程大约需要 30 分钟。平均淋巴引流为 0.71 +/- 0.33 ml/h，蛋白质浓度在 12 小时内没有明显变化（平均 37 +/- 2.59 mg/ml）。然而，淋巴细胞的数量在 0.08 +/- 0.03 x 10(6)/ml 和 12.17 +/- 6.58 x 10(6)/ml 之间波动很大。这种改良的胸导管淋巴采集动物模型避免了脂质摄入、全身麻醉或动物活动受限对实验结果的影响，因此更能准确反映正常生理条件下的淋巴流量和成分。
