Morgun V V, Larchenko E A, Kostianovskiĭ R G, Keterinchuk A M
Tsitol Genet. 2011 Jul-Aug;45(4):36-43.
The paper covers investigation of cytogenetic activity of chiral mutagens and their specific effects on the plant cells chromosomes of soft winter wheat (Triticum aestivum L.). Comparative analysis of cytogenetic activity of chiral NEU: S(+)1-N-nitroso- 1-N-methyl-3-N-sec-buthylureas (S(+)NMsBU) and R(-)1-N-nitroso- 1N-methyl-3-Nsec-buthylureas (R(-)NMsBU) on winter wheat was performed. As it was shown by the frequency of chromosomal aberrations the S(+) stereoisomer was twice more active than R(-). In addition to typical anaphase aberrations (fragments, bridges, lagging chromosomes) the numerous mitosis pathologies were revealed - K-mitoses, hyperspiralization and despiralization of chromosomes, unequal allocation of chromosomes between the daughter nuclei, mass fragmentation, nondisjunction and chromosome adhesion, three-pole mitoses, etc. Neither of the mentioned pathologies was observed under the action of NEU and gamma-rays.
该论文涵盖了对手性诱变剂的细胞遗传活性及其对软质冬小麦（Triticum aestivum L.）植物细胞染色体的特定影响的研究。对手性NEU：S(+)1-亚硝基-1-甲基-3-仲丁基脲（S(+)NMsBU）和R(-)1-亚硝基-1-甲基-3-仲丁基脲（R(-)NMsBU）对冬小麦的细胞遗传活性进行了比较分析。从染色体畸变频率来看，S(+)立体异构体的活性是R(-)的两倍。除了典型的后期畸变（片段、桥、滞后染色体）外，还发现了许多有丝分裂病理学现象——K-有丝分裂、染色体的超螺旋化和解螺旋化、子核间染色体的不均等分配、大量片段化、不分离和染色体粘连、三极有丝分裂等。在NEU和γ射线作用下未观察到上述任何病理学现象。
