Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago, Japan.
Hepatol Res. 2012 Jan;42(1):15-21. doi: 10.1111/j.1872-034X.2011.00874.x. Epub 2011 Sep 22.
Regenerative medicine using stem cells has attracted much attention, since stem cells are responsible for highly proliferative activity and multipotential ability of differentiation. Induced pluripotent stem cells and embryonic stem cells or the adult stem cells such as bone marrow-derived stem cells and adipose tissue-derived stem cells have been expected as a cell source of regenerative medicine. Since differentiating methods of human stem cells into the defined lineage of cells remains to be developed, we focus on the differentiating strategies of pluripotent stem cells and mesenchymal stem cells into liver lineage, especially on cytokine function and gene expression during hepatic differentiation. The survey of previously published papers discloses that the protocols that mimic the liver developmental process seem to be effective in obtaining functional hepatocytes. However, in order to develop hepatic regenerative medicine that is useful in a clinical setting, more effective and potent strategies that obtain mature hepatocytes are required.
再生医学使用干细胞已引起广泛关注，因为干细胞具有高度增殖活性和多能性分化能力。诱导多能干细胞和胚胎干细胞或成体干细胞如骨髓来源的干细胞和脂肪组织来源的干细胞已被期望作为再生医学的细胞来源。由于将人类干细胞分化为特定谱系细胞的方法仍有待开发，我们专注于多能干细胞和间充质干细胞向肝谱系的分化策略，特别是细胞因子功能和基因表达在肝分化过程中的作用。对已发表论文的调查表明，模拟肝发育过程的方案似乎在获得功能性肝细胞方面是有效的。然而，为了开发在临床环境中有用的肝再生医学，需要更有效和更强的获得成熟肝细胞的策略。
