Departments of Medicine Clinical Pathology, Saiseikai Kyoto Hospital, Nagaoka-kyo Molecular Gastroenterology and Hepatology, Kyoto, Japan.
Pathol Int. 2011 Oct;61(10):572-6. doi: 10.1111/j.1440-1827.2011.02743.x.
We previously reported that hepatocellular aging can be assessed by measuring the nuclear size of hepatocytes. We attempted to elucidate whether this method is useful to identify the high risk group of hepatocellular carcinoma (HCC) in the patients with non-B non-C non-alcoholic liver injury. Fourteen patients with HCC and 78 without HCC, both of whom presented with non-B non-C non-alcoholic chronic liver injury and underwent liver biopsy, were selected. Twelve histologically normal liver tissues were selected as controls. The relative nuclear size (RNS) was calculated as the average nuclear size of the hepatocytes divided by that of lymphocytes. Multiple clinicopathological parameters were studied. The RNS values of normal livers ranged from 1.32 to 2.10, showing a gradual increase in an age-dependent manner. The RNS values of the injured livers without HCC increased after middle age. Univariate analysis identified greater age, existence of diabetes and RNS, as significantly positive contributors and ALT value and the degree of steatosis as negative contributors for the occurrence of HCC. Only age and RNS retained significance in multivariate analysis. All of the HCC patients were older than 50 and showed RNS values higher than 2.00. Therefore, such patients are classified as a high risk group of HCC.
我们之前报道过，可以通过测量肝细胞的细胞核大小来评估肝细胞的衰老程度。我们试图阐明这种方法是否有助于识别非乙型肝炎、非丙型肝炎、非酒精性肝损伤患者中肝细胞癌（HCC）的高危人群。选择了 14 例 HCC 患者和 78 例无 HCC 患者，他们均患有非乙型肝炎、非丙型肝炎、非酒精性慢性肝损伤，并接受了肝活检。选择 12 例组织学正常的肝脏组织作为对照。相对核大小（RNS）的计算方法为肝细胞的平均核大小除以淋巴细胞的核大小。研究了多个临床病理参数。正常肝脏的 RNS 值范围为 1.32 至 2.10，呈年龄依赖性逐渐增加。无 HCC 的损伤肝脏的 RNS 值在中年后增加。单因素分析确定年龄较大、存在糖尿病和 RNS 以及 ALT 值和脂肪变性程度是 HCC 发生的显著正贡献因素，而 RNS 值和脂肪变性程度是 HCC 发生的显著负贡献因素。只有年龄和 RNS 在多因素分析中仍然具有统计学意义。所有 HCC 患者的年龄均大于 50 岁，且 RNS 值高于 2.00。因此，这些患者被归类为 HCC 的高危人群。
