Kashara T, Yamaguchi Y, Yamashita K
Department of Medical Biology and Parasitology, Jichi Medical School, Tochigi-pref.
Rinsho Byori. 1990 Apr;38(4):380-5.
Current features of interleukins and cytokines are summarized with emphasis laid on their biological significance. Based on the biological activities of these cytokines, a variety of bioassays for the detection and quantitation methods have been developed. Simple and useful bioassays using cytokine-dependent cell lines are introduced. In particular, bioassays on IL 1, IL 2, IL 4, IL 5 and IL 6 are illustrated in detail. Recent developments on the various immunoassays (ELISA and RIA), which are more specific to each cytokine, are reported. In addition, the problems and limitations of these assay methods are pointed out.
本文总结了白细胞介素和细胞因子的当前特征，并重点阐述了它们的生物学意义。基于这些细胞因子的生物学活性，已开发出多种用于检测和定量的生物测定方法。本文介绍了使用依赖细胞因子的细胞系进行简单而有用的生物测定方法。特别详细说明了针对白细胞介素1、白细胞介素2、白细胞介素4、白细胞介素5和白细胞介素6的生物测定方法。报道了对每种细胞因子更具特异性的各种免疫测定法（酶联免疫吸附测定法和放射免疫测定法）的最新进展。此外，还指出了这些测定方法存在的问题和局限性。
