Kotani T, Ohtaki S
Central Laboratory for Clinical Investigation, Miyazaki Medical College Hospital.
Rinsho Byori. 1990 Apr;38(4):442-5.
Thyroid Peroxidase (TPO) is a key enzyme in the synthesis of thyroid hormone and is a major thyroid microsomal antigen corresponding to anti-microsomal autoantibodies in thyroid autoimmune diseases. We studied the autoantigenicity, thyroiditogenicity and gene structure of TPO. In micro-ELISA using human TPO as a target, all sera from patients with anti-microsomal antibodies contained IgG class of antibodies to TPO and some sera had IgM class of antibodies. The competitive inhibition test revealed that TPO is the major thyroid microsomal antigen. Experimental murine thyroiditis was successfully induced by the immunization of porcine TPO. Susceptibility of thyroiditis in each strain was very different from that of thyroiditis induced by thyroglobulin. T-cell line specific for porcine TPO could mediate thyroid lesions. Two kinds of full length cDNAs to human TPO were isolated from cDNA library which was constructed from mRNA purified from thyroid with Graves' disease. The longer one consisted of 3,048 nucleotides and its open-reading-frame was likely to encode 933 amino acids. The shorter one lacked 171 nucleotides at the middle portion of the longer one. The structure-gene for human TPO was located on 2q and consisted of 17 exons. One hundred and seventy-one nucleotides deleted in the shorter cDNA exactly corresponded to the 10th exon.
甲状腺过氧化物酶（TPO）是甲状腺激素合成中的关键酶，是甲状腺自身免疫性疾病中与抗微粒体自身抗体相对应的主要甲状腺微粒体抗原。我们研究了TPO的自身抗原性、致甲状腺炎性和基因结构。在以人TPO为靶点的微量ELISA中，所有抗微粒体抗体患者的血清均含有针对TPO的IgG类抗体，部分血清含有IgM类抗体。竞争抑制试验表明TPO是主要的甲状腺微粒体抗原。通过猪TPO免疫成功诱导了实验性小鼠甲状腺炎。各品系甲状腺炎的易感性与甲状腺球蛋白诱导的甲状腺炎有很大差异。针对猪TPO的T细胞系可介导甲状腺病变。从Graves病甲状腺纯化的mRNA构建的cDNA文库中分离出两种人TPO全长cDNA。较长的一种由3048个核苷酸组成，其开放阅读框可能编码933个氨基酸。较短的一种在较长的一种中间部分缺失171个核苷酸。人TPO的结构基因位于2q，由17个外显子组成。较短cDNA中缺失的171个核苷酸正好对应于第10个外显子。
