College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Biochem Genet. 2012 Apr;50(3-4):227-34. doi: 10.1007/s10528-011-9464-2. Epub 2011 Sep 28.
The Na(+)/H(+) exchanger 1 (NHE1) transmembrane protein regulates intracellular pH, cell survival, cell growth, cell differentiation and plays a critical role in the progression of some diseases, including the pathogenesis of J avian leukosis. The chicken is an ideal model to study the function of NHE1 because it has developed highly efficient Na(+)-absorptive mechanisms in its small and large intestines. To date, there has been no detailed expression analysis to determine NHE1 expression in various tissues of the chicken. We determined the mRNA and protein expression levels of avian NHE1 by real-time quantitative PCR and immunohistochemical analysis. NHE1 mRNA was detected in all chicken tissues examined. Protein expression levels varied widely among tissues and did not always correlate with mRNA expression. Determining the mRNA and protein of NHE1 expression patterns in chicken should help to delineate the NHE1 role in different tissues and its contribution to physiological and pathological processes. These data provide the basis for examining the distinct function of chicken NHE1 compared with its mammalian counterpart.
钠/氢交换蛋白 1（NHE1）跨膜蛋白调节细胞内 pH 值、细胞存活、细胞生长、细胞分化，在一些疾病的进展中发挥着关键作用，包括禽白血病的发病机制。鸡是研究 NHE1 功能的理想模型，因为它在小肠和大肠中已经发展出非常高效的钠离子吸收机制。迄今为止，还没有详细的表达分析来确定鸡体内各种组织中 NHE1 的表达情况。我们通过实时定量 PCR 和免疫组织化学分析来确定禽类 NHE1 的 mRNA 和蛋白表达水平。在检测的所有鸡组织中均检测到 NHE1 mRNA。蛋白表达水平在组织间差异很大，并不总是与 mRNA 表达相关。确定鸡 NHE1 的 mRNA 和蛋白表达模式有助于阐明 NHE1 在不同组织中的作用及其对生理和病理过程的贡献。这些数据为研究鸡 NHE1 与哺乳动物 NHE1 的不同功能提供了基础。
