College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Theor Appl Genet. 2012 Feb;124(2):287-93. doi: 10.1007/s00122-011-1704-7. Epub 2011 Sep 28.
The English grain aphid, Sitobion avenae (Fabricius), is one of the most important insect pests causing substantial yield losses in wheat production in China and other grain-growing areas in the world. The efficient utilization of wheat genes for resistance to English grain aphid (EGA) provides an efficient, economic and environmentally sound approach to reduce the yield losses. In the present study, the wheat line C273 (Triticum durum AABB, 2n = 4x = 28), is resistant to EGA in greenhouse and field tests. To identify the resistance gene, designated RA-1 temporarily, C273 was crossed with susceptible genotype Poland 305 (T. polonicum, AABB, 2n = 4x = 28). The F(1), F(2) and F(2:3) lines were tested with EGA in the field and greenhouse. The results indicated that RA-1 is a single dominant gene, closely linked to the microsatellite markers (SSR) Xwmc179, Xwmc553 and Xwmc201 on chromosome 6AL at genetic distances of 3.47, 4.73 and 7.57 cM, respectively. The three SSR markers will be valuable in marker-assisted selection for resistance to EGA as well as for cloning this gene in the future.
麦长管蚜是造成中国乃至世界其他粮食产区小麦减产的重要害虫之一。利用小麦抗蚜基因是减少产量损失的有效、经济和环保的方法。本研究中，温室和田间试验表明，小麦品系 C273（硬粒小麦 AABB，2n=4x=28）对麦长管蚜具有抗性。为了鉴定该抗性基因，暂命名为 RA-1，将 C273 与易感基因型波兰 305（波兰小麦 AABB，2n=4x=28）进行杂交。将 F(1)、F(2)和 F(2:3)代在田间和温室中用麦长管蚜进行测试。结果表明，RA-1 是一个单一的显性基因，与 6AL 染色体上的微卫星标记（SSR）Xwmc179、Xwmc553 和 Xwmc201 紧密连锁，遗传距离分别为 3.47、4.73 和 7.57cM。这三个 SSR 标记将有助于对麦长管蚜抗性进行标记辅助选择，也有助于将来克隆该基因。
Zotero 插件