Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India.
Phytother Res. 2012 Apr;26(4):568-78. doi: 10.1002/ptr.3586. Epub 2011 Sep 26.
The present study demonstrated the potential antimetastatic and antiinvasive effect of berberine using both in vivo mouse lung metastasis and in vitro models. Administration of berberine resulted in significant suppression of B16F-10 melanoma induced tumor nodule formation and enhanced the survival of tumor-bearing mice. Berberine treatment also decreased various biochemical parameters associated with lung metastasis. These inhibitory actions may be due to the significant suppression of several signaling molecules such as ERK1/2, NF-κB, ATF-2 and CREB involved in the transcription signaling pathways for MMP gene expression. It could also inhibit the migration and invasion of highly metastatic murine melanoma cells in a dose-dependent manner in vitro. The results clearly show that berberine could significantly inhibit experimental lung metastasis produced by intravenous injection of B16F-10 melanoma cells and this effect could be linked to the down-regulation of metastasis-related signaling molecules.
本研究采用体内小鼠肺转移模型和体外模型证实了小檗碱的潜在抗肿瘤转移和抗侵袭作用。小檗碱的给药显著抑制了 B16F-10 黑色素瘤诱导的肿瘤结节形成，并提高了荷瘤小鼠的存活率。小檗碱处理还降低了与肺转移相关的多种生化参数。这些抑制作用可能是由于显著抑制了几种信号分子，如 ERK1/2、NF-κB、ATF-2 和 CREB，这些信号分子参与了 MMP 基因表达的转录信号通路。小檗碱还可以体外剂量依赖性地抑制高转移性鼠黑色素瘤细胞的迁移和侵袭。结果清楚地表明，小檗碱可以显著抑制静脉注射 B16F-10 黑色素瘤细胞引起的实验性肺转移，这种作用可能与下调转移相关的信号分子有关。
