Wei Ming, Zhang Jin-Jun, He Qiu-Lan, Wang Lei, Ren Zheng-Hua, Sun Lai-Bao, Liu Xian-Guo
Department of Anesthesiology, First-affiliated Hospital, Guangzhou 510080, China.
Zhong Yao Cai. 2011 May;34(5):746-50.
To study the effects of osthole on sciatica induced by lumber disc herniation and its mechanisms.
54 male SD rats were randomly divided into 6 groups. Model (M) group (n = 12): Autologous nucleus pulposus was harvested from the tail and applied to the L5 dorsal root ganglion (DRG) and epidural space. Epidural catheterization was performed. Control(C) group (n = 12): On the basis of nucleus pulposus group, 50 microL tween-80 was administered epidurally on the day 6th after surgery. T2 (n = 6), T6 (n = 12), T13 (n = 6) and T20 (n = 6) group: 50 microL 2% osthole was administered epidurally on the 2th, 6th, 13th day and 20th after surgery respectively. General behaviors were observed and 50% paw withdrawal threshold (50% PWT) was measured 1 day before surgery, on the 1st, 3th, 7th,14th, 21th, 28th day after surgery, immediately before and 1 hour after osthole or tween-80 administration in each group. On the 7th day after surgery, the left L5 DRGs were obtained for detecting the expression of NOS and COX-2 in M, C and T6 group with 6 rats.
No lameness or autophagy was oberserved. 50% PWT decreased after surgery (P < 0.05). In T2 and T6 group, 50% PWT after osthole administration were significantly higher than those of M group and C group (P < 0.05), which recovered to the same level as 1 day before surgery (P > 0.05). In T13 and T20 group, 50% PWT 1 hour after osthole administration were significantly higher than those of M group and C group (P < 0.05), which recovered to the same level as 1 day before surgery (P > 0.05), but on days after 1 hour after administration, there was no significant difference when 50% PWT compared with M group or nucleus C group (P > 0.05). NOS positive cells and COX-2 positive cells were no significant difference when M group compared with C group (P > 0.05). But these positive cells in T6 group were significantly lower than those of M group and C group (P < 0.05).
50 microL 2% osthole could completely inhibit the mechanical allodynia in the rat model of sciatica induced by lumbar disc herniation when it was administered epidurally on 2 or 6 day after surgery. But when administered on 13 or 20 day after surgery, its analgesic effect was transient. The effect of 50 microL 2% osthole epidural administration on day 6 after surgery on the rat model of sciatica induced by lumbar disc herniation may relate to inhibition of the expression of COX-2 and NOS in DRG.
研究蛇床子素对腰椎间盘突出症所致坐骨神经痛的影响及其作用机制。
将54只雄性SD大鼠随机分为6组。模型（M）组（n = 12）：自大鼠尾部获取自体髓核，将其置于L5背根神经节（DRG）及硬膜外间隙，并进行硬膜外导管置入。对照（C）组（n = 12）：在髓核组基础上，于术后第6天硬膜外给予50 μL吐温-80。T2（n = 6）、T6（n = 12）、T13（n = 6）和T20（n = 6）组：分别于术后第2、6、13、20天硬膜外给予50 μL 2%蛇床子素。观察一般行为表现，并于术前1天、术后第1、3、7、14、21、28天，以及每组给予蛇床子素或吐温-80前即刻及给药后1小时测量50%缩爪阈值（50% PWT）。术后第7天，取左侧L5 DRG，每组6只大鼠，检测M、C和T6组中一氧化氮合酶（NOS）和环氧化酶-2（COX-2）的表达。
未观察到跛行或自噬现象。术后50% PWT降低（P < 0.05）。在T2和T6组中，给予蛇床子素后50% PWT显著高于M组和C组（P < 0.05），并恢复至术前1天水平（P > 0.05）。在T13和T20组中，给予蛇床子素1小时后50% PWT显著高于M组和C组（P < 0.05），并恢复至术前1天水平（P > 0.05），但给药后1小时后的各天，与M组或C组比较50% PWT无显著差异（P > 0.05）。M组与C组比较，NOS阳性细胞和COX-2阳性细胞无显著差异（P > 0.05）。但T6组这些阳性细胞显著低于M组和C组（P < 0.05）。
术后2或6天硬膜外给予50 μL 2%蛇床子素可完全抑制腰椎间盘突出症所致坐骨神经痛大鼠模型的机械性异常疼痛。但术后13或20天给药时，其镇痛作用是短暂的。术后第6天硬膜外给予50 μL 2%蛇床子素对腰椎间盘突出症所致坐骨神经痛大鼠模型的作用可能与抑制DRG中COX-2和NOS的表达有关。
