Iudinkova E S, Bunina D A, Ul'ianov S V, Gavrilov A A, Razin S V
Mol Biol (Mosk). 2011 Jul-Aug;45(4):662-7.
Using native chromatin immunoprecipitation (N-ChIP) followed by TaqMan RT-PCR quantitative analysis we have determined the profiles of histone acetylation and histone methylation within the alpha-globin gene domain before and after switching of embryonic globin genes expression. The results obtained do not support a supposition that the inactivation of the embryonic alpha-type globin gene pi in erythroid cells of the adult lineage is mediated via formation of an inactive chromatin domain. On the other hand we have demonstrated that suppression of the gene pi activity in erythroid cells of adult lineage correlates with the decrease of the histone acetylation level within the embryonic subdomain of the alpha-globin gene domain.
通过使用原生染色质免疫沉淀（N-ChIP）并结合TaqMan RT-PCR定量分析，我们确定了胚胎珠蛋白基因表达转换前后α-珠蛋白基因结构域内组蛋白乙酰化和组蛋白甲基化的图谱。所得结果不支持这样一种假设，即成年谱系红细胞中胚胎α型珠蛋白基因π的失活是通过形成无活性染色质结构域介导的。另一方面，我们已经证明，成年谱系红细胞中基因π活性的抑制与α-珠蛋白基因结构域胚胎亚结构域内组蛋白乙酰化水平的降低相关。
