Eliseev B D, Alkalaeva E Z, Kriuchkova P N, Lekomtsev S A, Wang Wei, Liang Ai-hua, Frolova L Iu
Mol Biol (Mosk). 2011 Jul-Aug;45(4):668-72.
We have determined the type of stop codon specificity of Blepharisma japonicum translation termination factor eRF1 in an in vitro reconstituted eukaryotic translation system and in in vivo assay (the dual reporter system). We have shown that B. japonicum eRF1 retained specificity towards all three stop codons although efficiency of peptydyl-tRNA hydrolysis in the presence of UGA is reduced in an in vitro assay. We suggest that since the heterotrich B. japonicum represents the earliest diverged lineage on phylogenetic tree of ciliates, B. japonicum has the universal genetic code as ancestor group for all ciliates.
我们已经在体外重建的真核翻译系统和体内试验（双报告系统）中确定了日本棘尾虫翻译终止因子eRF1的终止密码子特异性类型。我们已经表明，尽管在体外试验中，UGA存在时肽基-tRNA水解的效率降低，但日本棘尾虫eRF1对所有三个终止密码子仍保持特异性。我们认为，由于异毛纲的日本棘尾虫代表了纤毛虫系统发育树中最早分化的谱系，日本棘尾虫拥有作为所有纤毛虫祖先群体的通用遗传密码。
