Department of Urology, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
J Exp Clin Cancer Res. 2011 Sep 28;30(1):88. doi: 10.1186/1756-9966-30-88.
Protein kinase C epsilon (PKCε), an oncogene overexpressed in several human cancers, is involved in cell proliferation, migration, invasion, and survival. However, its roles in clear cell renal cell carcinoma (RCC) are unclear. This study aimed to investigate the functions of PKCε in RCC, especially in clear cell RCC, to determine the possibility of using it as a therapeutic target. By immunohistochemistry, we found that the expression of PKCε was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation, wound healing, and Borden assays showed that down-regulating PKCε by RNA interference resulted in inhibition of the growth, migration, and invasion of clear cell RCC cell line 769P and, more importantly, sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCε siRNA-transfected cells. These results indicate that the overexpression of PKCε is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease.
蛋白激酶 C ɛ（PKCε）是一种在多种人类癌症中过度表达的癌基因，参与细胞增殖、迁移、侵袭和存活。然而，其在透明细胞肾细胞癌（RCC）中的作用尚不清楚。本研究旨在探讨 PKCε 在 RCC 中的功能，特别是在透明细胞 RCC 中的作用，以确定将其作为治疗靶点的可能性。通过免疫组织化学，我们发现 PKCε 在 RCC 中的表达上调，并且与透明细胞 RCC 中的肿瘤 Fuhrman 分级和 T 分期相关。克隆形成、划痕愈合和 Borden 测定表明，通过 RNA 干扰下调 PKCε 导致透明细胞 RCC 细胞系 769P 的生长、迁移和侵袭受到抑制，更重要的是，PKCε siRNA 转染的细胞中 caspase-3 的活性增强，表明对化疗药物更敏感。这些结果表明，PKCε 的过表达与透明细胞 RCC 的侵袭表型相关，可能是该疾病的潜在治疗靶点。
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