DNA聚合酶α和δ在DNA复制中的作用。

The roles of DNA polymerases alpha and delta in DNA replication.

作者信息

Talanian R V, Wright G E

机构信息

Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.

出版信息

Pharmacol Ther. 1990;47(1):105-15. doi: 10.1016/0163-7258(90)90047-6.

DOI:10.1016/0163-7258(90)90047-6

PMID:2195552

Abstract

The identities and precise roles of the DNA polymerase(s) involved in mammalian cell DNA replication are uncertain. Circumstantial evidence suggests that DNA polymerase alpha and at least one form of DNA polymerase delta, that which is stimulated by Proliferating Cell Nuclear Antigen, catalyze mammalian cell replicative DNA synthesis. Further, the in vitro properties of polymerases alpha and delta suggest a model for their coordinate action at the replication fork. The present paper summarizes the current status of DNA polymerases alpha and delta in DNA replication, and describes newly available approaches to the study of those enzymes.

摘要

参与哺乳动物细胞DNA复制的DNA聚合酶的身份及其确切作用尚不确定。间接证据表明，DNA聚合酶α和至少一种形式的DNA聚合酶δ（即受增殖细胞核抗原刺激的DNA聚合酶δ）催化哺乳动物细胞复制性DNA合成。此外，聚合酶α和δ的体外特性提示了它们在复制叉处协同作用的模型。本文总结了DNA聚合酶α和δ在DNA复制中的当前研究状况，并描述了研究这些酶的新方法。